Electrophysiological properties of mouse horizontal cell GABAA receptors

Journal of Neurophysiology
A Feigenspan, R Weiler

Abstract

GABA-induced currents have been characterized in isolated horizontal cells from lower vertebrates but not in mammalian horizontal cells. Therefore horizontal cells were isolated after enzymatical and mechanical dissociation of the adult mouse retina and visually identified. We recorded from horizontal cell bodies using the whole cell and outside-out configuration of the patch-clamp technique. Extracellular application of GABA induced inward currents carried by chloride ions. GABA-evoked currents were completely and reversibly blocked by the competitive GABAA receptor antagonist bicuculline (IC50 = 1.7 microM), indicating expression of GABAA but not GABAC receptors. Their affinity for GABA was moderate (EC50 = 30 microM), and the Hill coefficient was 1.3, corresponding to two GABA binding sites. GABA responses were partially reduced by picrotoxin with differential effects on peak and steady-state current values. Zinc blocked the GABA response with an IC50 value of 7.3 microM in a noncompetitive manner. Furthermore, GABA receptors of horizontal cells were modulated by extracellular application of diazepam, zolpidem, methyl 6,7-dimethoxy-4-ethyl-beta-carboxylate, pentobarbital, and alphaxalone, thus showing typical pharmacological...Continue Reading

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Citations

Nov 11, 2008·Pflügers Archiv : European journal of physiology·Andreas FeigenspanReto Weiler
Jul 21, 2007·Visual Neuroscience·Arlene A HiranoNicholas C Brecha
Jul 31, 2007·Visual Neuroscience·Ping Li, Malcolm Slaughter
Jun 23, 2010·Neural Development·Timm SchubertRachel O L Wong
Jul 21, 2009·The European Journal of Neuroscience·Karin DedekReto Weiler
Feb 21, 2006·The European Journal of Neuroscience·H HayasakiM Watanabe
Jul 6, 2006·The European Journal of Neuroscience·Jennifer ShelleyReto Weiler
Aug 3, 2006·The European Journal of Neuroscience·Petra DirksDirk Montag
Nov 26, 2010·Journal of Neurochemistry·Sercan DenizMichel J Roux
Sep 12, 2012·The Journal of Comparative Neurology·Katharina KranzUlrike Janssen-Bienhold
Mar 18, 2010·The Journal of Comparative Neurology·Chenying GuoNicholas C Brecha
Apr 14, 2010·The European Journal of Neuroscience·Helen Lee, Nicholas C Brecha
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Dec 23, 2008·Experimental Eye Research·Sindri TraustasonEinar Stefánsson
Sep 25, 2014·Frontiers in Neural Circuits·Wadood HaqTimm Schubert
Jul 8, 2016·International Journal of Molecular Sciences·Francisco Andrés Peralta, Juan Pablo Huidobro-Toro
Jun 14, 2013·Epilepsy & Behavior : E&B·Dana Craiu
Apr 2, 2019·PLoS Biology·James C R GroveSteven Barnes
Jun 2, 2006·Journal of Neurophysiology·Timm SchubertAndreas Feigenspan
Nov 11, 2016·Journal of Neurophysiology·Michael W Country, Michael G Jonz
Dec 19, 2020·Frontiers in Cellular Neuroscience·Arlene A HiranoNicholas C Brecha
Dec 18, 2020·Frontiers in Cellular Neuroscience·Steven BarnesNicholas C Brecha

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