Electrophysiological properties of three types of granulocytes in circulating blood of the newt

The Journal of Physiology
K Kawa

Abstract

1. The electrophysiological properties of three subtypes of granulocytes obtained from the circulating blood of the newt, Triturus pyrrhogaster, were studied using the whole-cell variation of the patch-electrode voltage-clamp technique. 2. Neutrophils were identified by their multilobulated nucleus in the cytoplasm. Basophils and eosinophils, having characteristic granular structures in their cells, could definitely be distinguished from other leucocytes. The reliability of cellular identification using phase-contrast microscopy was confirmed by fixing and staining the granulocytes with Wright's solution. 3. In neutrophils under current-clamp conditions, a hyperpolarization-induced conductance increase was observed. With depolarization, however, no changes in regenerative potential were detected. When voltage clamped in standard saline (containing 96 mM-NaCl), hyperpolarizing voltage pulses to a potential more negative than -90 mV evoked slowly decaying inward currents. 4. The hyperpolarization-evoked membrane currents in neutrophils were identified as anomalous rectifying K+ currents, since (1) externally applied Cs+ (0.1 or 1 mM) or Ba2+ (1 mM) produced suppressive effects on the currents, (2) replacement of external Na+ with...Continue Reading

Citations

Mar 10, 1998·The Journal of Physiology·M TareT B Bolton
Dec 19, 2017·Acta Neuropathologica·Golo KronenbergKaren Gertz
Dec 5, 2014·American Journal of Physiology. Cell Physiology·Ricard MasiaGary Yellen
May 3, 2018·Pflügers Archiv : European journal of physiology·Karolina NajderBenedikt Fels
Jan 1, 1992·The American Journal of Physiology·M MukaiM Kuno
Dec 12, 1991·Biochimica Et Biophysica Acta·M Lindau, B D Gomperts

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