Electrophysiological recordings from the Drosophila giant fiber system (GFS).

Cold Spring Harbor Protocols
Marcus J Allen, Tanja A Godenschwege

Abstract

The giant fiber system (GFS) of Drosophila is a well-characterized neuronal circuit that mediates the escape response in the fly. It is one of the few adult neural circuits from which electrophysiological recordings can be made routinely. This protocol describes a simple procedure for stimulating the giant fiber neurons directly in the brain of the adult fly and obtaining recordings from the output muscles of the GFS: the tergotrochanteral "jump" muscle (TTM) and the large indirect flight muscles (dorsal longitudinal muscles, or DLMs). It is a relatively noninvasive method that allows the investigator to stimulate the giant fibers in the brain and assay the function of several central synapses within this neural circuit by recording from the thoracic musculature.

References

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Dec 28, 2005·Seminars in Cell & Developmental Biology·Marcus J AllenPauline Phelan
Jan 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Huaiyu Gu, Diane K O'Dowd
Jan 13, 2006·Current Biology : CB·Tanja A GodenschwegeRodney K Murphey
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Jul 4, 2008·The Journal of Biological Chemistry·Melanie R WatsonNancy M Bonini
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May 29, 2009·Journal of Neurophysiology·Haleh FotowatFabrizio Gabbiani

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Citations

Jun 16, 2011·Journal of Visualized Experiments : JoVE·Jana Boerner, Tanja A Godenschwege
Nov 30, 2014·Molecular and Cellular Neurosciences·LaTasha H Lee, Tanja A Godenschwege
Nov 1, 2011·Methods : a Companion to Methods in Enzymology·Christopher J H Elliott, John C Sparrow
Aug 21, 2010·Toxicon : Official Journal of the International Society on Toxinology·Monica MejiaTanja A Godenschwege
Jun 14, 2016·Cell Chemical Biology·Paloma Navas-NavarroJavier García-Sancho
Jul 30, 2019·Frontiers in Cellular Neuroscience·Tyrone PensergaTanja Angela Godenschwege
May 16, 2019·Disease Models & Mechanisms·Mireia Coll-TanéAnnette Schenck
May 2, 2018·Journal of Neuroscience Methods·David P GoodmanCatherine R von Reyn
Dec 16, 2020·Biochemical and Biophysical Research Communications·Björn Brembs

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