Electrophysiological retinal pigment epithelium changes observed with indocyanine green, trypan blue and triamcinolone

Ophthalmic Research
Carl ArndtLars-Olof Hattenbach

Abstract

Intravitreal application of indocyanine green (ICG), trypan blue (TB) or triamcinolone (TA) during vitreoretinal surgery has been associated with severe damage to the retinal pigment epithelium (RPE). However, the physiological background of these findings remains to be assessed. In bovine RPE choroid preparations maintained in Ussing chambers, the effect of apical application of ICG, TA (filtered and not filtered) and TB at different concentrations was evaluated. The electrophysiological parameters (transepithelial potential, tissular resistance and short-circuit current) were continuously monitored. The experiments were conducted either in daylight or in dark-adapted conditions. After apical application of ICG and TA (purified and not purified), all bioelectrical parameters were affected in a dose-dependent manner. No significant changes were observed when the preparations were exposed to daylight. No changes were observed with TB. The electrophysiological results of apical application may explain the toxic effects observed after intraoperative use of ICG and TA. In this RPE study, TB appears to be the safest visualization aid for vitreoretinal surgery.

Citations

Jul 31, 2013·Acta Ophthalmologica·Zengping LiuUNKNOWN International Chromovitrectomy Collaboration
Oct 23, 2013·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde
Dec 22, 2012·Ophthalmic Research·Dicle Hazirolan, Uwe Pleyer
Dec 11, 2019·Journal français d'ophtalmologie·C CouretB Bodaghi

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