Electrophysiology of embryonic, adult and aged rat hippocampal neurons in serum-free culture

Journal of Neuroscience Methods
M S EvansGregory J Brewer

Abstract

Methods were recently developed for culturing neurons from adult rat hippocampus using the serum-free medium Neurobasal with B27 supplement. To determine whether adult cultured neurons have normal electrical properties, we studied cultures from rats of three age groups: (1) embryonic; (2) 10-11 months old and (3) 35-36 months old. Neurons had a polarized morphology with a large branching apical dendrite and small basal dendrites. Mean resting potentials were similar in the three age groups. All neurons had nonlinear current-voltage relationships, indicating the presence of voltage-sensitive ion channels. Most neurons had a voltage-sensitive inward current followed by a sustained voltage-sensitive outward current. Tetrodotoxin blocked the inward current, which is likely to be a sodium current. The sustained outward current, which is likely to be a potassium current, reversed at -71 mV. Most neurons exhibited anomalous rectification. Calcium currents were present in both embryonic and adult neurons. Embryonic neurons would sometimes fire multiple action potentials but adult neurons fired only single action potentials. Our results indicate that both embryonic and adult cultured neurons retain a clearly neuronal electrophysiologica...Continue Reading

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Citations

May 8, 2001·Journal of Neuroscience Research·J J VielG J Brewer
Jan 9, 2007·Journal of Materials Science. Materials in Medicine·J MaQ Y Xu
Aug 22, 2001·European Journal of Pharmacology·N Stella, D Piomelli
Jun 8, 2001·Journal of Neuroscience Methods·G J BrewerD C McManus
Jul 9, 2002·Journal of Neuroscience Methods·Claire WyartDidier Chatenay
Oct 30, 2003·Journal of Neuroscience Methods·Jill K LeutgebThomas Behnisch
Apr 21, 1999·Brain Research Bulletin·M A CollingsM S Evans
Dec 13, 2000·Experimental Gerontology·G J Brewer
Sep 27, 2012·Nature Communications·Kwang-Mook JungOlivier J Manzoni
Sep 21, 2012·Journal of Neurophysiology·William F BorschelGabriela K Popescu
Mar 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sung W ChoiMartin D Brand
May 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·D FegleyD Piomelli
Oct 29, 2008·Journal of Neuroscience Methods·Kucku VargheseJames J Hickman
Jan 18, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M F Cano-AbadA Ruiz-Nuño
Nov 20, 2012·Progress in Neuro-psychopharmacology & Biological Psychiatry·Hyunjeong KimKee Namkoong
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Jun 15, 2011·The Journal of Cell Biology·Mei-Yao LinRuey-Hwa Chen

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