PMID: 6968907Jul 1, 1980Paper

Electrophysiology of T lymphocyte cholinergic receptors

Proceedings of the National Academy of Sciences of the United States of America
H M Shapiro, T B Strom

Abstract

The presence of functional muscarinic-cholinergic receptors on at least some T lymphocytes is suggested by the existence of saturable, high-affinity, specific muscarinic binding in T cell-enriched, but not in B cell-enriched, cell suspensions and by observed cholinergic effects on lymphocytes, (e.g., increased lytic capacity of cytotoxic lymphocytes preincubated with muscarinic agents). In this study, we used flow cytometry and a fluorescent probe of membrane potential, the cyanine dye 3,3'-dihexyloxacarbocyanine iodide, to examine the effects of cholinergic agonists and antagonists on the membrane potentials of lymphocytes in T cell-enriched and B cell-enriched suspensions. Acetylcholine (AcCho) and carbamoylcholine (CbmCho) depolarized the membranes of T cells, but not of B cells; the maximal depolarization was produced by 10 nM AcCho or by 1 nM CbmCho. Depolarization following exposure to these concentrations of agonists was maximal by 5-8 min; T cell membrane potentials returned to control values by 13-15 min. Less marked depolarization was produced by 100 nM AcCho and 10 nM CbmCho; 100 pM CbmCho was only slightly less effective than 1 nM CbmCho, and the depolarization persisted 12 min after exposure. Depolarization induced...Continue Reading

References

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Citations

Apr 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·A D AdoV I Dontsov
Jan 1, 1983·Molecular Aspects of Medicine·N R FaridJ C Bear
Oct 1, 1992·International Journal of Immunopharmacology·A M GenaroE Borda
Oct 19, 2007·Journal of the American Society of Nephrology : JASN·Terry B Strom
Nov 1, 1985·Journal of Neuroimmunology·T L Roszman, W H Brooks
Jun 1, 1993·Journal of Neuroimmunology·H B SolvasonR N Hiramoto
May 1, 1990·Clinical Immunology and Immunopathology·G GorelikE S Borda
Feb 7, 1998·Primary Care·D R Chiarmonte
Sep 15, 1982·Analytical Biochemistry·H S Kruth
Oct 1, 1983·Journal of Cellular Physiology·K S Rosenthal, H M Shapiro
Jul 1, 1982·The American Journal of Physiology·S H AppelT Ashizawa

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