PMID: 3758827Sep 1, 1986Paper

Electrophysiology of the human colon: evidence of segmental heterogeneity

Gut
G I SandleH J Binder

Abstract

The electrical properties of epithelial cell membranes in human descending and ascending colon were studied using microelectrodes and the Na channel blocker amiloride. Under control (pre-amiloride) conditions, the transepithelial electrical measurements in the two colonic segments were similar. The mucosal addition of 0.1 mM amiloride to descending colon totally abolished the transepithelial voltage (Vt) and short-circuit current (Isc), and significantly increased the total tissue resistance (Rt) by 19% (p less than 0.005). Intracellular recordings in descending colon obtained with microelectrodes revealed that the transepithelial effects of amiloride reflected hyperpolarization of the apical membrane and a significant increase in apical membrane resistance, changes which were consistent with amiloride-blockade of apical Na channels and complete inhibition of electrogenic Na transport. An appreciable amiloride-insensitive conductance was also present in the apical membrane of the descending colon. In contrast, the mucosal addition of 0.1 mM amiloride to ascending colon decreased Vt by only 30% (p less than 0.02) and Isc by 43% (p less than 0.05), but had no significant effect on Rt. These results indicate that electrogenic Na t...Continue Reading

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Citations

Aug 20, 2010·Inflammatory Bowel Diseases·Klaudia FarkasPéter Hegyi
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