PMID: 12193376Aug 24, 2002

Electroporated transgene-rescued spermatogenesis in infertile mutant mice with a sertoli cell defect

Biology of Reproduction
Kentaro YomogidaYoshitake Nishimune

Abstract

The molecular basis of most human male infertility arising from spermatogenesis disruption is poorly understood because of a lack of useful investigation systems. To study the roles of the supporting Sertoli cells in mammalian spermatogenesis, we improved an electroporation technique for seminiferous tubules in vivo. Because Sertoli cells barely proliferate in mature testis, linear transgenes are not incorporated into the genome and quickly degrade. However, circular expression vector is stably expressed in Sertoli cells for a long period. By electrotransformation of a complete cDNA, we rescued defective spermatogenesis in infertile Sl(17H)/Sl(17H) mutant mice with partial dysfunction of stem cell factor in Sertoli cells. Application of this gene transfer system will facilitate both the understanding of spermatogenesis and the development of new gene therapies for human male infertility.

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Citations

Oct 15, 2008·Nature Clinical Practice. Urology·Kim Boekelheide, Mark Sigman
Jan 19, 2011·Nature Reviews. Urology·Kathleen HwangLarry I Lipshultz
Jun 11, 2004·Biology of Reproduction·M Kanatsu-ShinoharaT Shinohara
Dec 7, 2007·Biology of Reproduction·HuangHui TangErwin Goldberg
May 17, 2008·Development, Growth & Differentiation·Kentaro Yomgogida
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Apr 11, 2012·Acta Histochemica Et Cytochemica·Takami TakizawaToshihiro Takizawa
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Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea
Jan 10, 2016·Fertility and Sterility·Kathrin Gassei, Kyle E Orwig
Dec 19, 2012·Spermatogenesis·Hye-Won Song, Miles F Wilkinson
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji
Oct 20, 2014·Histochemistry and Cell Biology·Lei DaiTakehiko Koji
Aug 12, 2005·Experimental Animals·Hasbaira BolorNoboru Wakasugi
May 28, 2005·Human Reproduction·M Kanatsu-ShinoharaT Shinohara
Feb 5, 2004·Journal of Andrology·Mary A Bedell, Aparna Mahakali Zama
Dec 28, 2017·Human Reproduction Update·João Pedro Alves-Lopes, Jan-Bernd Stukenborg
Feb 17, 2005·Journal of Andrology·Yukihiro UmemotoKenjiro Kohri

Related Concepts

Isoactin
Cell Differentiation Process
DNA, Circular
Subfertility, Male
Luminescent Proteins
Mice, Inbred C57BL
Early Promoters, Genetic
Seminiferous Tubule Structure
Structure of Sertoli Cell
Finding of Sperm Number

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