Electroporatic delivery of TGF-beta1 gene works synergistically with electric therapy to enhance diabetic wound healing in db/db mice

The Journal of Investigative Dermatology
Pui-Yan LeeLeaf Huang

Abstract

Electrical stimulation (ES) is a therapeutic treatment for wound healing. Electroporation, a type of ES, is a well-established method for gene delivery. We hypothesize that proper conditions can be found with which both electrical and gene therapies can be additively applied to treat diabetic wound healing. For the studies of transforming growth factor-beta1 (TGF-beta1) local expression and therapeutic effects, full thickness excisional wound model of db/db mice was used, we measured TGF-beta1 cytokine level at 24 h postwounding and examined wounds histologically. Furthermore, wound closure was evaluated by wound-area measurements at each day for 14 d. We found that syringe electrodes are more effective than the conventional caliper electrodes. Furthermore, diabetic skin was more sensitive to the electroporative damage than the normal skin. The optimal condition for diabetic skin was six pulses of 100 V per cm for 20 ms. Under such condition, the healing rate of electrically treated wound was significantly accelerated. Furthermore, when TGF-beta1 gene was delivered by electric pulses, the healing rate was further enhanced. Five to seven days postapplication of intradermal injection of plasmid TGF-beta1 followed by electroporati...Continue Reading

References

Jan 17, 1991·Biochimica Et Biophysica Acta·A V TitomirovE Kistanova
Mar 1, 1996·Journal of Dental Research·J A GarlickL B Taichman
Apr 1, 1996·Biological & Pharmaceutical Bulletin·M OkumuraM Yajima
Sep 22, 1998·Nature Biotechnology·H Aihara, J Miyazaki
Apr 7, 1999·Progress in Retinal and Eye Research·J V JesterH D Cavanagh
Jan 13, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·S E GardnerF L Schmidt
Apr 28, 2000·Annals of Biomedical Engineering·M R ChoD E Golan
Jul 7, 1997·Advanced Drug Delivery Reviews·R HellerM J Jaroszeski
Jun 6, 2000·Advanced Drug Delivery Reviews·U Pliquett
Jun 6, 2000·Advanced Drug Delivery Reviews·R HellerM J Jaroszeski
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·F Yao, E Eriksson
Mar 10, 2001·DNA and Cell Biology·R HellerC Nicolau
Sep 18, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R M GallucciM I Luster
Jan 10, 2002·The Journal of Cell Biology·Jo Ellen FeugateManuela Martins-Green
Feb 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Feng Liu, Leaf Huang
Mar 29, 2002·Journal of Cellular Physiology·Peter Ten DijkeSusumu Itoh
Nov 27, 2002·Cell Motility and the Cytoskeleton·Françoise DéclinePatricia Rousselle
Feb 19, 2003·Experimental Eye Research·Entong WangColin D McCaig

❮ Previous
Next ❯

Citations

Dec 6, 2008·Lasers in Medical Science·Kenan CinarNurgul Senol
Jan 14, 2009·Expert Reviews in Molecular Medicine·Leena PradhanAristidis Veves
Jan 12, 2007·Gene Therapy·G J Prud'hommeQ Wang
Jan 8, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lars SteinstraesserGaëlle Vandermeulen
Oct 30, 2015·Expert Opinion on Biological Therapy·Laure Gibot, Marie-Pierre Rols
May 22, 2013·Expert Opinion on Emerging Drugs·Francesco TecilazichAristidis Veves
Sep 21, 2007·Expert Opinion on Drug Delivery·Yoshitaka Isaka, Enyu Imai
Nov 22, 2005·Expert Opinion on Drug Delivery·Loree C HellerRichard Heller
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Huihui LiuShanyu Guo
Jul 3, 2008·International Wound Journal·Ramin Mostofi Zadeh Farahani
Jul 3, 2008·International Wound Journal·Ramin Mostofizadeh Farahani, Luther C Kloth
Jan 19, 2011·Expert Opinion on Pharmacotherapy·Francesco TecilazichAristidis Veves
Apr 4, 2012·Journal of Ethnopharmacology·Xiao-bo WuJia-hong Xu
Jun 12, 2013·Advanced Drug Delivery Reviews·Shanmugamurthy LakshmananMichael R Hamblin
Sep 11, 2007·Medical Hypotheses·Ramin Mostofi Zadeh FarahaniLuther C Kloth
Jan 29, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Karen H L KwanKenneth K Y Wong
Oct 12, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tanja BlagusGregor Sersa
Apr 5, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Pui Yan LeeLeaf Huang
Feb 18, 2017·ACS Applied Materials & Interfaces·Qiankun ZhangKyung Mi Woo
Aug 31, 2016·Experimental Dermatology·John W SessionsBrian D Jensen
Jul 24, 2013·Journal of Burn Care & Research : Official Publication of the American Burn Association·Marc G JeschkeUNKNOWN ABA Organization and Delivery of Burn Care Committee
Aug 29, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Gérald J Prud'homme
Aug 25, 2006·Gene Therapy·L K BranskiM G Jeschke
Feb 6, 2020·Cells·Sergio LiarteFrancisco J Nicolás
Sep 7, 2018·Frontiers in Pharmacology·Yaw D BoakyeKwabena O Danquah
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Ashang Luwang LaivaMichael B Keogh
Jan 1, 2010·Expert Review of Endocrinology & Metabolism·Jason P GlotzbachGeoffrey C Gurtner
Dec 24, 2010·The Journal of Veterinary Medical Science·Ayman AtibaYuji Uzuka
Dec 31, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Calver PangMallappa K Kolar
Sep 30, 2018·Advanced Drug Delivery Reviews·Austin P VeithAaron B Baker
May 9, 2007·Journal of Dermatological Science·Zhen GaoWei Liu
Aug 23, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Omid YazarluMaede Hasanpour

❮ Previous
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