Electroporation-Based Genetic Manipulation in Type I Methanotrophs

Applied and Environmental Microbiology
Xin YanMary E Lidstrom

Abstract

Methane is becoming a major candidate for a prominent carbon feedstock in the future, and the bioconversion of methane into valuable products has drawn increasing attention. To facilitate the use of methanotrophic organisms as industrial strains and accelerate our ability to metabolically engineer methanotrophs, simple and rapid genetic tools are needed. Electroporation is one such enabling tool, but to date it has not been successful in a group of methanotrophs of interest for the production of chemicals and fuels, the gammaproteobacterial (type I) methanotrophs. In this study, we developed electroporation techniques with a high transformation efficiency for three different type I methanotrophs: Methylomicrobium buryatense 5GB1C, Methylomonas sp. strain LW13, and Methylobacter tundripaludum 21/22. We further developed this technique in M. buryatense, a haloalkaliphilic aerobic methanotroph that demonstrates robust growth with a high carbon conversion efficiency and is well suited for industrial use for the bioconversion of methane. On the basis of the high transformation efficiency of M. buryatense, gene knockouts or integration of a foreign fragment into the chromosome can be easily achieved by direct electroporation of PCR-g...Continue Reading

References

Jul 1, 1992·Applied and Environmental Microbiology·D W GrahamR G Arnold
May 1, 1970·Journal of General Microbiology·R WhittenburyJ F Wilkinson
Jun 1, 1996·Microbiological Reviews·R S Hanson, T E Hanson
Nov 27, 2002·BioTechniques·Christopher J Marx, Mary E Lidstrom
Jan 24, 2004·Nucleic Acids Research·Nikolai A ShevchukStephan Ladisch
Apr 9, 2008·Advances in Applied Microbiology·Yuri A Trotsenko, John Colin Murrell
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mohamed Baani, Werner Liesack
Jul 22, 2008·Applied and Environmental Microbiology·Xin YanShun-Peng Li
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
May 21, 2013·Environmental Microbiology·Jeremy D SemrauJ Colin Murrell
Jul 3, 2013·Genome Announcements·Valentina N KhmeleninaMarina G Kalyuzhnaya
Sep 21, 2013·ACS Synthetic Biology·François St-PierreKeith E Shearwin
Apr 15, 2014·Biotechnology Advances·Qiang FeiPhilip T Pienkos
May 7, 2014·Antimicrobial Agents and Chemotherapy·Audrey BernutLaurent Kremer
Nov 8, 2014·Nucleic Acids Research·Richard J RobertsDana Macelis
Dec 31, 2014·Applied and Environmental Microbiology·Aaron W PuriMary E Lidstrom
Feb 28, 2015·Environmental Science & Technology·P J StrongW P Clarke
Mar 15, 2015·Genome Announcements·Marina G KalyuzhnayaLudmila Chistoserdova
Apr 1, 2015·Metabolic Engineering·Marina G KalyuzhnayaMary E Lidstrom

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Citations

Jul 2, 2016·Journal of the American Chemical Society·Thomas J Lawton, Amy C Rosenzweig
Mar 2, 2018·Applied Microbiology and Biotechnology·In Yeub HwangEun Yeol Lee
Jan 20, 2018·The Journal of Biological Chemistry·Grace E Kenney, Amy C Rosenzweig
Jan 7, 2018·Applied and Environmental Microbiology·Jeremy D SemrauSukhwan Yoon
Dec 21, 2016·Journal of Bacteriology·Aaron W PuriMary E Lidstrom
Sep 30, 2018·Applied and Environmental Microbiology·Masahito IshikawaKatsutoshi Hori
Apr 19, 2018·Annual Review of Biochemistry·Grace E Kenney, Amy C Rosenzweig
Jan 12, 2019·Annual Review of Biochemistry·Rahul BanerjeeJohn D Lipscomb
Jan 7, 2017·Science·James M ClomburgRamon Gonzalez
Mar 29, 2018·MBio·Yue ZhengLudmila Chistoserdova
Apr 17, 2020·Frontiers in Microbiology·Yongchuang LiuXin Yan
Nov 22, 2020·Applied and Environmental Microbiology·Lian HeMary E Lidstrom
Jan 26, 2021·Chemical Society Reviews·Christopher W Koo, Amy C Rosenzweig
Aug 24, 2020·Trends in Biotechnology·Anh Duc Nguyen, Eun Yeol Lee
Apr 24, 2021·Scientific Reports·Olga N RozovaIldar I Mustakhimov

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