Electroporation loading of membrane-impermeable molecules to investigate intra- and intercellular Ca2+ signaling

Cold Spring Harbor Protocols
Elke DecrockLuc Leybaert

Abstract

Electroporation is a technique that temporarily induces pores in the plasma membranes of cells, thereby allowing plasma membrane-impermeable substances to enter the cells. This loading method requires an electrical drive circuit providing an electroporation signal, an electrode to apply the signal to a localized zone in a cell monolayer, and a special solution that has a low electrical conductivity. To avoid impairment of cell function and cell death from the electroporation procedure itself, the applied electrical signal should ideally be a high-frequency oscillating signal (50 kHz) without any direct current (DC) component. Here, we describe the detailed procedure of electroporation loading.

Citations

Jan 13, 2018·Frontiers in Physiology·Nunzio VicarioRosalba Parenti
Mar 4, 2015·Cold Spring Harbor Protocols·Elke DecrockLuc Leybaert
Mar 4, 2015·Cold Spring Harbor Protocols·Elke DecrockLuc Leybaert

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