Electroporation of Brain Endothelial Cells on Chip toward Permeabilizing the Blood-Brain Barrier

Biophysical Journal
Mohammad BonakdarRafael V Davalos

Abstract

The blood-brain barrier, mainly composed of brain microvascular endothelial cells, poses an obstacle to drug delivery to the brain. Controlled permeabilization of the constituent brain endothelial cells can result in overcoming this barrier and increasing transcellular transport across it. Electroporation is a biophysical phenomenon that has shown potential in permeabilizing and overcoming this barrier. In this study we developed a microengineered in vitro model to characterize the permeabilization of adhered brain endothelial cells to large molecules in response to applied pulsed electric fields. We found the distribution of affected cells by reversible and irreversible electroporation, and quantified the uptaken amount of naturally impermeable molecules into the cells as a result of applied pulse magnitude and number of pulses. We achieved 81 ± 1.7% (N = 6) electroporated cells with 17 ± 8% (N = 5) cell death using an electric-field magnitude of ∼580 V/cm and 10 pulses. Our results provide the proper range for applied electric-field intensity and number of pulses for safe permeabilization without significantly compromising cell viability. Our results demonstrate that it is possible to permeabilize the endothelial cells of the...Continue Reading

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Citations

Dec 18, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Elena Sánchez-LópezMaria Luisa García
Sep 22, 2018·Technology in Cancer Research & Treatment·Daniel C SweeneyRafael V Davalos
Aug 30, 2017·Annals of Biomedical Engineering·Elisa M WassonRafael V Davalos
Jul 5, 2019·Nature Nanotechnology·Héctor Acarón LedesmaBozhi Tian
Nov 17, 2016·Biophysical Journal·Tomo MurovecChristian Brosseau
May 28, 2021·Electromagnetic Biology and Medicine·Sahel HeydarheydariZeinab Shankayi
May 16, 2021·Journal of Biomechanical Engineering·Zheng FangBing Zhang
Jul 23, 2021·Advanced Science·Elise P W JenkinsGeorge G Malliaras

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