Electroporation of mammalian cells by nanosecond electric field oscillations and its inhibition by the electric field reversal

Scientific Reports
Elena C GianulisAndrei G Pakhomov

Abstract

The present study compared electroporation efficiency of bipolar and unipolar nanosecond electric field oscillations (NEFO). Bipolar NEFO was a damped sine wave with 140 ns first phase duration at 50% height; the peak amplitude of phases 2-4 decreased to 35%, 12%, and 7% of the first phase. This waveform was rectified to produce unipolar NEFO by cutting off phases 2 and 4. Membrane permeabilization was quantified in CHO and GH3 cells by uptake of a membrane integrity marker dye YO-PRO-1 (YP) and by the membrane conductance increase measured by patch clamp. For treatments with 1-20 unipolar NEFO, at 9.6-24 kV/cm, 10 Hz, the rate and amount of YP uptake were consistently 2-3-fold higher than after bipolar NEFO treatments, despite delivering less energy. However, the threshold amplitude was about 7 kV/cm for both NEFO waveforms. A single 14.4 kV/cm unipolar NEFO caused a 1.5-2 times greater increase in membrane conductance (p<0.05) than bipolar NEFO, along with a longer and less frequent recovery. The lower efficiency of bipolar NEFO was preserved in Ca2+-free conditions and thus cannot be explained by the reversal of electrophoretic flows of Ca2+. Instead, the data indicate that the electric field polarity reversals reduced the p...Continue Reading

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Citations

Sep 17, 2016·Scientific Reports·Vitalij NovickijDamijan Miklavčič
Dec 18, 2016·Cellular and Molecular Life Sciences : CMLS·Elena C GianulisAndrei G Pakhomov
Apr 23, 2017·Biochimica Et Biophysica Acta. Biomembranes·Caterina MerlaP Thomas Vernier
Nov 29, 2017·Scientific Reports·Chris M ValdezBennett L Ibey
Mar 3, 2018·Journal of Biophotonics·Aniket ChowdhuryShovan K Majumder
Sep 13, 2019·Scientific Reports·Elena C GianulisAndrei G Pakhomov
Sep 7, 2017·Scientific Reports·Maura CasciolaAndrei G Pakhomov
Sep 30, 2018·Scientific Reports·Vitalij NovickijIrutė Girkontaitė
May 15, 2020·International Journal of Molecular Sciences·Wenfei BoAndrei G Pakhomov
Sep 15, 2020·Bioelectrochemistry·Rosa OrlacchioPhilippe Leveque
Apr 15, 2019·Biochimica Et Biophysica Acta. Biomembranes·Esin B Sözer, P Thomas Vernier
May 8, 2018·Bioelectrochemistry·Shu XiaoSambasiva R Rajulapati
Jul 3, 2021·International Journal of Molecular Sciences·Vitalii KimAndrei G Pakhomov
Mar 1, 2020·Bioelectricity·Gen UrabeSunao Katsuki
Oct 24, 2020·Biophysical Journal·Esin B SözerJoshua Zimmerberg

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

pCLAMP
Amaze
MetaMorph Advanced
Digidata

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