Electroporation of pp60c-src antibodies inhibits the angiotensin II activation of phospholipase C-gamma 1 in rat aortic smooth muscle cells.

The Journal of Biological Chemistry
M B MarreroK E Bernstein

Abstract

Our previous study has shown that angiotensin II induces the rapid tyrosine phosphorylation and activation of phospholipase C-gamma 1 in cultured rat aortic smooth muscle (RASM) cells (Marrero, M.B., Paxton, W.G., Duff, J. L., Berk, B. C., and Bernstein, K. E. (1994) J. Biol. Chem, 269, 10935-10939). This signaling pathway is initiated by ligand binding to the AT1 receptor, a cell surface G protein-coupled receptor. Antibodies to pp60c-src were introduced into RASM cells by electroporation. Angiotensin II-stimulated tyrosine phosphorylation of phospholipase C-gamma 1 was eliminated by the anti-pp60c-src antibodies but not by anti-mouse IgG or bovine serum albumin. Angiotensin II also induced the rapid tyrosine phosphorylation of pp120, a known pp60c-src kinase substrate, and this phosphorylation was also specifically inhibited by anti-pp60c-src antibodies. Electroporation of RASM cells with anti-pp60c-src antibodies had no effect on platelet-derived growth factor-stimulated tyrosine phosphorylation of PLC-gamma 1. Anti-pp60c-src also reduced the angiotensin II-stimulated inositol 1,4,5-trisphosphate production by 78%, while it had no effect on the platelet-derived growth factor-stimulated inositol 1,4,5-trisphosphate production...Continue Reading

References

Apr 11, 1991·American Journal of Hypertension·A T ChiuP B Timmermans
Apr 11, 1991·American Journal of Hypertension·P C WongP B Timmermans
Feb 1, 1991·Trends in Pharmacological Sciences·P B TimmermansW F Herblin
Mar 1, 1991·American Journal of Hypertension·P B BottiniL M Prisant
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Mar 31, 1993·Biochemical and Biophysical Research Communications·F LiaoS G Rhee
Nov 1, 1993·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·K E Bernstein, B C Berk

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Citations

Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K FukudaY Matsuzawa
Mar 18, 2000·Biochemical and Biophysical Research Communications·M SanoS Ogawa
Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Verónica González Pardo, Ana Russo de Boland
Nov 4, 2000·Regulatory Peptides·J Haendeler, B C Berk
Sep 1, 2000·Regulatory Peptides·K K Griendling, M Ushio-Fukai
Jan 8, 1999·Regulatory Peptides·P P SayeskiK E Bernstein
Aug 1, 1996·Trends in Cardiovascular Medicine·K E Bernstein, M B Marrero
Oct 20, 2001·Cell Research·D F GuoT Inagami
Aug 5, 1998·Kidney International·A V CybulskyA J McTavish
Nov 14, 2000·Molecular and Cellular Biology·R A BlakeS A Courtneidge
Jan 1, 1997·Annual Review of Biochemistry·W D SingerP C Sternweis
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Jul 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hiroshi NozawaSufi M Thomas
Jul 28, 2010·Stem Cell Research & Therapy·Jennifer C MooreRonald P Hart
Aug 20, 2003·Journal of Neurosurgery·Gen KusakaJohn H Zhang
Feb 13, 2001·Biochemical and Biophysical Research Communications·M LuchtefeldB Schieffer
Jan 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Armon ShareiKlavs F Jensen
Apr 3, 2002·The Journal of Cell Biology·Guangju JiMichael I Kotlikoff
Apr 16, 2015·PloS One·Armon ShareiKlavs F Jensen
Oct 3, 1999·The Journal of Biological Chemistry·A F GiustiL A Jaffe
Oct 3, 2003·The Journal of Biological Chemistry·Judith HaendelerBradford C Berk
Jul 16, 1999·Clinical and Experimental Pharmacology & Physiology·T InagamiT Yamakawa
Oct 16, 1999·Circulation Research·T ToyofukuM Hori

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