Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture

BioMed Research International
Olga MichelAdam Rzechonek

Abstract

Despite the rapid progression of cancer pharmacotherapy, the high drug resistance of pancreatic ductal adenocarcinoma (PDA) makes it one of the most lethal malignancies. Therefore, there are high expectations associated with experimental therapies, such as electrochemotherapy (ECT). This technique involves the application of short electric pulses to induce transitional permeabilization of the cellular membrane, thus enhancing drug molecules influx. The aim of the study was to investigate the influence of electroporation with cisplatin (CisEP) on the primary culture of human PDA cells from lung metastases-their survival and stress response. Considering the growing importance of various research models, two established human PDA cell lines, EPP85-181P (sensitive to daunorubicin) and EPP85-181RDB (resistant to daunorubicin), were utilized as a reference control. Cisplatin revealed higher cytotoxicity towards established cell lines. Following CisEP application, we observed a significant decrease of cells viability in the primary culture model. After CisEP therapy, an increased immunoreactivity with SOD-2 and Casp-3 antibodies was noticed. In conclusion, we discovered that electroporation can enhance the cytotoxic effect of cisplati...Continue Reading

References

Jan 11, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L M MirC Paoletti
Jan 1, 1991·Archives of Toxicology·J HayG Laurent
Aug 15, 1990·Hearing Research·D McAlpine, B M Johnstone
May 26, 1998·Japanese Journal of Cancer Research : Gann·M CemazarG Sersa
Jun 1, 2000·Anti-cancer Drugs·M J JaroszeskiR Heller
May 30, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M CemazarG Sersa
Mar 31, 2006·The Kaohsiung Journal of Medical Sciences·Yue-Chiu SuChee-Yin Chai
Jul 13, 2006·IEEE Transactions on Bio-medical Engineering·Jon F EddBoris Rubinsky
Oct 18, 2006·International Journal of Cancer. Journal International Du Cancer·Maria BerndtssonStig Linder
Jan 24, 2007·Technology in Cancer Research & Treatment·Boris RubinskyPaul Mikus
Jul 7, 2007·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·G SersaM Snoj
Feb 26, 2008·Folia Histochemica Et Cytobiologica·Jolanta SaczkoHonorata Krawczykowska
Nov 7, 2008·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yolanda I Chirino, José Pedraza-Chaverri
Oct 3, 2009·Taiwanese Journal of Obstetrics & Gynecology·Fu-Shing Liu
Oct 27, 2009·Biochemical and Biophysical Research Communications·Liling TangCaixin Sun
Dec 4, 2009·PloS One·P Thomas VernierD Peter Tieleman
Sep 9, 2010·Neoplasia : an International Journal for Oncology Research·Wolfgang HagmannJohannes Matthias Löhr
Dec 8, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hee-Sun YangKwon-Il Seo
Jan 14, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Stefania MeschiniEnrico P Spugnini
Mar 17, 2012·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Richard FischerJan Harder
May 9, 2012·Free Radical Biology & Medicine·Sanjit Kumar Dhar, Daret K St Clair
Sep 24, 2014·The Journal of Urology·Juan Luis VásquezJulie Gehl
Apr 29, 2015·International Journal of Surgery·V GranataF Izzo
Jun 2, 2015·Radiology and Oncology·Roberto GirelliBassi Claudio
Jul 1, 2015·International Journal of Surgery·Salvatore TafutoUNKNOWN ENETS Center of Excellence Multidisciplinary Group for Neuroendocrine Tumors in Naples (Italy)
Nov 19, 2015·Acta Pharmaceutica Sinica. B·Xiangming Guan
Jun 18, 2016·The Journal of Membrane Biology·Stine Krog FrandsenJulie Gehl
Jul 20, 2017·Annals of Biomedical Engineering·Tyler MiklovicMichael B Sano

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Citations

Sep 11, 2019·Technology in Cancer Research & Treatment·Ying ChenBing Zhang
Sep 3, 2019·Biotechnology and Applied Biochemistry·Olga MichelAdam Rzechonek
Oct 21, 2020·International Journal of Molecular Sciences·Chang-Min LeeByung-Hoon Kim
Jun 9, 2020·ACS Applied Materials & Interfaces·Changhao LiChengyun Ning
Dec 1, 2019·Bioelectricity·Maja Cemazar, Gregor Sersa
Dec 7, 2021·Photochemistry and Photobiology·Nicholas P ToupinJeremy J Kodanko

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Methods Mentioned

BETA
biopsy
flow cytometry
FACS

Software Mentioned

Excel
GraphPad Prism
Statistica

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