Electroporative alpha-MSH gene transfer attenuates thioacetamide-induced murine hepatic fibrosis by MMP and TIMP modulation

Gene Therapy
C-H WangBruno Jawan

Abstract

Hepatic fibrosis represents a process of healing and scarring in response to chronic liver injury. alpha-Melanocyte-stimulating hormone (alpha-MSH) is a 13-amino-acid peptide with potent anti-inflammatory effects. We have previously demonstrated that alpha-MSH gene therapy protects against thioacetamide (TAA)-induced acute liver failure. Therefore, the aim of this study is to investigate whether alpha-MSH gene therapy possesses antihepatic fibrogenic effect. Liver fibrosis was induced by long-term TAA administration in mice. alpha-Melanocyte-stimulating hormone expression plasmid was delivered via electroporation after liver fibrosis was established. Our results showed that alpha-MSH gene therapy attenuated liver fibrosis in TAA-treated mice. Reverse transcription polymerase chain reaction revealed that alpha-MSH gene therapy attenuated the liver transforming growth factor-beta1, collagen alpha1 and cell adhesion molecule mRNA upregulation. Following gene transfer, the expression of alpha-smooth muscle actin and cyclooxygenase-2 were both significantly attenuated. Further, alpha-MSH significantly increased matrix metalloproteinase (MMP), while tissue inhibitors of matrix metalloproteinase (TIMPs) were inactivated. In summary, a...Continue Reading

References

Jul 1, 1992·Gastroenterology·H TilgC Huber
Jul 1, 1980·The Journal of Endocrinology·J F Wilson, F M Harry
Jul 1, 1995·Biological Chemistry Hoppe-Seyler·M KissK Scharffetter-Kochanek
May 1, 1996·The Journal of Clinical Investigation·H ChiaoR A Star
Dec 10, 1998·The American Journal of Pathology·A E KossakowskaS J Urbanski
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M J Arthur
Sep 9, 2000·Molecular Aspects of Medicine·G Poli
Sep 21, 2000·Trends in Endocrinology and Metabolism : TEM·A CataniaJ M Lipton
Aug 30, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M F DonatoM Colombo
Oct 5, 2001·Seminars in Liver Disease·D SchuppanE G Hahn
Mar 19, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Axel M GressnerSteven Dooley
Mar 19, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Helen L Reeves, Scott L Friedman
Jun 28, 2003·Journal of Cell Science·Giovanni Maga, Ulrich Hubscher
Jul 10, 2003·Annals of the New York Academy of Sciences·Thomas A LugerMarkus Böhm
Oct 8, 2003·Current Pharmaceutical Design·Joan Clària
Dec 3, 2003·The Journal of Biological Chemistry·Markus BöhmThomas A Luger
Apr 2, 2004·Gastroenterology·Fernando Siller-LópezJuan Armendariz-Borunda
Apr 13, 2004·European Journal of Gastroenterology & Hepatology·János SchnurSnorri S Thorgeirsson
Apr 20, 2004·Journal of Hepatology·Valeer J Desmet, Tania Roskams
May 4, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Emilia IpIsabelle Leclercq
Aug 19, 2004·Journal of Biomedical Science·Cheng-Haung WangJong-Kang Liu
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maria J MolnarGeorge Karpati
Sep 25, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christopher J ParsonsDavid A Brenner
Mar 15, 2005·Scandinavian Journal of Gastroenterology·J A Girón-GonzálezL Martín-Herrera
May 18, 2005·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Isaac TúnezPedro Montilla
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gualtiero ColomboAnna Catania
Aug 30, 2005·Biochemical and Biophysical Research Communications·Kung-Sheng HungCheng-Haung Wang

❮ Previous
Next ❯

Citations

Sep 16, 2006·Human Gene Therapy·Loree C Heller, Richard Heller
May 26, 2012·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Areeba Ahmad, Riaz Ahmad
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Grant J LoganGeorge C Yeoh
Jun 28, 2011·The American Journal of Pathology·Trinidad Montero-MelendezMauro Perretti
Jul 19, 2018·Clinical and Experimental Pharmacology & Physiology·Hussam Al-HumadiCharis Liapi
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vladimir BlagaićFilip Culo
Mar 9, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Tatsuya OhyamaMasatomo Mori
Jan 13, 2021·Pharmaceuticals·Roshan Dinparastisaleh, Mehdi Mirsaeidi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.