Electropreconcentration, gate injection, and capillary electrophoresis separation on a microchip

Journal of Chromatography. a
Honggu Chun

Abstract

The nanochannel-based electropreconcentration is not compatible with successive capillary zone electrophoresis (CZE). In this study, the incompatibility is theoretically discussed and experimentally proven, and then, the development of a monolithic glass microfluidic chip for performing integrated electropreconcentration and CZE separation is described. The sample is electropreconcentrated at the interface of a micro- and nanochannel where electric double layer overlap conditions exist. Because an ion-depletion region develops at the leading front of the preconcentrated plug, a field-enhanced sample stacking effect occurs which limits the separation efficiency unless compensated for. The ion-depletion region was confirmed by monitoring the solution conductivity at discrete points in the microchannel during the preconcentration step. The solution conductivity decreased >20-fold during the preconcentration step. To overcome the effects of this region, a cross-intersection was used to shunt the ion-depleted buffer away from the analysis channel while reintroducing the running buffer. When the preconcentrated sample plug arrives at the cross-intersection, it is gate injected into the analysis channel so that fresh running buffer su...Continue Reading

Citations

Sep 18, 2020·Advances in Colloid and Interface Science·Shixiang LiuHailin Cong

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