PMID: 9430618Feb 28, 1998Paper

Electrorotation of isolated generative and vegetative cells, and of intact pollen grains of Lilium longiflorum

The Journal of Membrane Biology
V L SukhorukovU Zimmermann

Abstract

The dielectric structure of mature pollen of the angiosperm Lilium longiflorum was studied by means of single-cell electrorotation. The use of a microstructured four-electrode chamber allowed the measurements to be performed over a wide range of medium conductivity from 3 to 500 mS m-1. The rotation spectra of hydrated pollen grains exhibited at least three well-resolved peaks in the kHz-MHz frequency range, which obviously arise due to the multilayered structure of pollen grains. The three-shell model can explain the complex rotational behavior of pollen grains in terms of conductivities, permittivities and thicknesses of the following compartments: the exine and intine of the pollen grain wall as well as the membrane and cytoplasm of the vegetative cell. However, the number of unknown parameters (more than 8) was too large to allow unambiguous values to be assigned to any of them. Therefore, to facilitate the evaluation of the pollen grain parameters, additional rotational measurements were made on isolated vegetative and generative cells. The rotation spectra of these cells could be fitted very accurately on the basis of the single-shell model by assuming a dispersion of the cytoplasm. The data on the membrane and cytoplasmi...Continue Reading

Citations

Sep 5, 2002·Electrophoresis·Peter R C Gascoyne, Jody Vykoukal
Dec 26, 2001·Biochimica Et Biophysica Acta·M KriegmaierV L Sukhorukov
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P K HeplerA Y Cheung
Jan 1, 2004·Proceedings of the IEEE·Peter R C Gascoyne, Jody V Vykoukal

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