PMID: 8587932Nov 1, 1995Paper

Electrorotational studies of the cytoplasmic dielectric properties of Friend murine erythroleukaemia cells

Physics in Medicine and Biology
Y HuangP R Gascoyne

Abstract

Electrorotation (ROT) spectra of Friend murine erythroleukaemia DS19 cells were measured in the frequency range 10 kHz-100 MHz as a function of suspension osmolality and cell differentiation treatment with hexamethylene bisacetamide (HMBA). A minimization program was employed to curve-fit the measured spectra using a single-shell dielectric model, allowing the derivation of cellular interior conductivity and permittivity (valid for the frequency range 10-100 MHz) and the cytoplasmic membrane capacitance (its dependence on the cell differentiation state and suspension osmolality having been reported earlier). Following HMBA treatment, DS19 cells exhibited a slight increment in average interior permittivity and a decrement in interior conductivity, although the changes were not statistically significant. For both untreated and HMBA treated samples, the average interior conductivity increased and permittivity decreased with increasing suspension osmolality. Of significance was that the average permittivity of cell interiors was larger than that of pure water. The electrorotation spectra of freshly prepared cell nuclei were measured, and the derived nuclear dielectric parameters were employed in numerical simulations to investigate...Continue Reading

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Citations

Sep 5, 2002·Electrophoresis·Peter R C Gascoyne, Jody Vykoukal
Jul 7, 2009·Analytical and Bioanalytical Chemistry·C ZhangK Kalantar-Zadeh
Jan 8, 1998·Biochimica Et Biophysica Acta·K L ChanR Pethig
Jun 3, 2009·IET Nanobiotechnology·R Hölzel
Jun 23, 2011·PLoS Computational Biology·Benjamin P CossinsVictor Guallar
Mar 26, 2014·Cancers·Peter R C Gascoyne, Sangjo Shim
Dec 12, 2012·Journal of Tissue Engineering and Regenerative Medicine·A IsmailF H Labeed
Nov 26, 2009·IEEE Transactions on Bio-medical Engineering·Alzbeta E HartingerHervé Gagnon
Feb 14, 2008·IEEE Transactions on Bio-medical Engineering·Ahmad Keshtkar, Asghar Keshtkar
Jan 1, 2004·Proceedings of the IEEE·Peter R C Gascoyne, Jody V Vykoukal
Jul 26, 2005·Journal of Chromatography. a·Carlos F Gonzalez, Vincent T Remcho
Jun 17, 2014·Lab on a Chip·Prateek BenhalWenhui Wang
Dec 14, 2018·Scientific Reports·Azita FazelkhahDouglas Thomson
Mar 6, 2002·Physiological Measurement·D C WalkerD M Jones
Jan 5, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Rino TakeuchiTomoyuki Yasukawa
Mar 17, 1999·Biochimica Et Biophysica Acta·Y HuangF F Becker
Jul 3, 2021·Membranes·Vanesa Viviana Galassi, Natalia Wilke
Jun 6, 2014·Chemical Reviews·Francois-Xavier TheilletPhilipp Selenko
Dec 14, 2011·Analytical Chemistry·Fabrice GielenJoshua B Edel
Aug 7, 2019·ACS Sensors·Ablaikhan Akhazhanov, Chi On Chui

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