Electrospinning of in situ crosslinked recombinant human collagen peptide/chitosan nanofibers for wound healing

Biomaterials Science
Aipeng DengShulin Yang

Abstract

Electrospun collagen nanofibers are effective for wound healing; however, many problems, such as the tedious preparation process, weak strength and poor structure integration, limit further applications. In this study, recombinant human collagen (RHC) peptides and a simple one-step crosslinking strategy were used to prepare RHC/chitosan nanofibers. With the nonpathogenic, water-soluble RHC and a mild electrospinning solvent, in situ crosslinked nanofibers (S-CN) not only simplified the preparation procedure but also maintained a more integrated morphology. Compared with the immersed crosslinked nanofibers (I-CN), S-CN showed better performance in moisture retention, degradation and mechanical strength tests. In vitro cell proliferation, morphology and RT-PCR studies confirmed that fibroblasts presented better activities on nanofibers crosslinked in situ. Importantly, after treating with the nanofibers, rapid epidermidalization and angiogenesis were observed in an SD rat scalding model. All these data suggest that electrospun RHC/chitosan nanofibers crosslinked in situ are an ideal candidate that can be used for wound healing applications.

References

May 24, 2003·Biomaterials·X H WangMark van der Werf
May 12, 2005·Biomaterials·Narayan BhattaraiMiqin Zhang
Sep 16, 2006·The Journal of Investigative Dermatology·Manon C ZweersBeate Eckes
Feb 13, 2007·Biomacromolecules·Jessica D Schiffman, Caroline L Schauer
Dec 29, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Y WangD D Fan
Jan 28, 2009·ACS Applied Materials & Interfaces·Sergio Torres-GinerJose M Lagaron
May 12, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Denys J LoeffelbeinMarco R Kesting
Apr 23, 2013·Journal of Biomedical Materials Research. Part a·Soumi Dey SarkarSantanu Dhara
Apr 24, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Haytham ElgharablyChandan K Sen
Apr 29, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiuli HuXiabin Jing
Aug 27, 2015·Journal of Pharmaceutical Sciences·Joshua Boateng, Ovidio Catanzano
Mar 30, 2016·Progress in Polymer Science·Fa-Ming Chen, Xiaohua Liu
Jul 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Fang ChengJohn E Eriksson
Sep 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Katrina A RiegerJessica D Schiffman
Jul 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·E Mele

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Citations

Feb 10, 2019·Journal of Biomedical Materials Research. Part a·Jinfei HouZhenxing Wang
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nan PanBotao Song
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Eoghan J Mulholland
Mar 14, 2020·Nature Communications·Yang WangYaojin Wang
Jan 1, 2020·Carbohydrate Polymers·Fenghua TaoHongbing Deng
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Yang YangNicola M Everitt
Apr 4, 2021·Bioengineering·Britani N BlackstoneHeather M Powell

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