Electrospray ionization Fourier transform ion cyclotron resonance at 9.4 T

Rapid Communications in Mass Spectrometry : RCM
M W SenkoA G Marshall

Abstract

We present the first results from a new electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer operated at a magnetic field of 9.4 T (i.e. > or = 2.4 T higher than for any prior FTICR instrument). The 9.4 T instrument provides substantially improved performance for large molecules (> or = 50% increase in mass resolving power) and complex mixtures (> or = 100% increase in dynamic range) compared to lower-field (< or = 6 T) instruments. The higher magnetic field makes possible larger trapped-ion population without introduction of significant space--charge effects such as spectral peak shift and/or distortion, and coalescence of closely-spaced resonances. For bovine ubiquitin (8.6 kDa) we observe accurate relative isotopic abundances at a signal-to-noise ratio greater than 1000:1, whereas a complete nozzle-skimmer dissociation electrospray ionization (ESI) FTICR mass spectrum of bovine carbonic anhydrase (29 kDa) is achieved from a single scan with a signal-to-noise ratio of more than 250:1. Finally, we are able to obtain mass resolving power, m/delta m > 200,000, routinely for porcine serum albumin (67 kDa). The present performance guides further modifications of the instrument, which should lead to s...Continue Reading

Citations

Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joseph D MougousCarolyn R Bertozzi
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Oct 23, 1997·Protein Science : a Publication of the Protein Society·Z ZhangA G Marshall
Jun 25, 2004·Nucleic Acids Research·Jorge Fernandez-de-CossioToshifumi Takao
Jul 26, 2007·Chemical Reviews·Tao LiuRichard D Smith
Mar 21, 2008·Analytical Chemistry·Michael J RothNeil L Kelleher
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