PMID: 9535270Apr 16, 1998Paper

Electrospray ionization mass spectrometric study of the multiple intracellular monomeric and polymeric hemoglobins of Glycera dibranchiata

Journal of Protein Chemistry
B N GreenS N Vinogradov

Abstract

The intracellular hemoglobin (Hb) of the marine polychaete Glycera dibranchiata is comprised of two groups of globins differing in their primary structures and state of aggregation. About six electrophoretically and chromatographically distinct monomeric Hbs which have Leu as the distal residue, and an equal number of polymeric Hbs which have the usual distal His, have been identified to date. Deconvolution of the electrospray ionization mass spectra (ESI-MS) of the Hbs and of their carbamidomethylated, reduced, and reduced/carbamidomethylated forms, using a maximum entropy-based approach (MaxEnt), showed the presence of at least 18 peaks attributable to monomer Hbs (14,500-15,200 Da) and an approximately equal number of polymer Hb peaks (15,500-16,400 Da). Although the ratio of the monomer to polymer components in pooled Hb preparations remained constant at 60:40, Hb from individuals had generally less than 6 monomer and 6 polymer components; -2 of the 19 individuals appeared to be deficient in polymer Hbs. Taking into account possible fragmentations of the known monomeric and polymeric globin sequences, we estimate conservatively that there are 10 monomeric and an equal number of polymeric Hbs, the majority comprising a singl...Continue Reading

Citations

Aug 12, 2014·Biochemical Society Transactions·Robert J BeynonJane L Hurst
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov
Dec 31, 2003·Journal of the American Society for Mass Spectrometry·Brian N Green, Serge N Vinogradov
Sep 3, 2021·Chemical Reviews·Amber D Rolland, James S Prell

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