Electrospray ionization mass spectrometric approach of conformationally-induced metal binding to oligopeptides

European Journal of Mass Spectrometry
Manuela MurariuGabi Drochioiu

Abstract

Electrospray ionization mass spectrometry was used to measure the binding of copper and nickel ions to the newly synthesized model peptides H(2)N-AAAAHAAAAHAAAAHAAAA-COOH (P19-H5) and H(2)N-AAAHAAAHAAAHAAAAAAA-COOH (P19-H4). The affinity of histidine-containing peptides toward heavy metal ions proved to be related to the position of each histidine residue in the peptide sequence. In contrast to P19-H5, P19-H4 peptide bound no nickel or copper ions in the gas phase, whereas its spectra showed an intense fragmentation. The role of spacing residues (Ala repeats) in selecting the various conformations was also investigated. Finally, the circular dichroism and Fourier transform infrared spectra indicated that these isomer peptides have quite different conformations. A close relationship between the conformation of alanine-based peptides and their affinity toward metal ions may result in different patterns of metal ion-peptide systems.

References

Dec 1, 1996·Neurodegeneration : a Journal for Neurodegenerative Disorders, Neuroprotection, and Neuroregeneration·J B Davis
Mar 15, 2000·Protein Science : a Publication of the Protein Society·R M WhittalM A Baldwin
Jun 9, 2001·Biochemical and Biophysical Research Communications·D Bar-OrC G Curtis
Feb 6, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Anthony R WhiteRoberto Cappai
Dec 14, 2004·Chemistry : a European Journal·Eliza-Diana CiuculescuPeter Faller
May 9, 2006·Biochemical and Biophysical Research Communications·K GaraiS Maiti
Jul 6, 2006·Trends in Biochemical Sciences·Kevin J BarnhamAndrew F Hill
Jul 29, 2006·Journal of the American Society for Mass Spectrometry·Juma D BridgewaterRichard W Vachet
Nov 23, 2006·Journal of Alzheimer's Disease : JAD·Paul A Adlard, Ashley I Bush
Jun 15, 2007·Isotopes in Environmental and Health Studies·Karin PopaGabi Drochioiu
Nov 29, 2007·The Journal of Biological Chemistry·Mark KlewpatinondJohn H Viles
Jan 15, 2008·European Journal of Mass Spectrometry·Gitta SchlosserGabi Drochioiu
Feb 29, 2008·Protein Science : a Publication of the Protein Society·Kwasi AntwiRichard W Vachet
Mar 18, 2008·Current Opinion in Chemical Biology·Kevin J Barnham, Ashley I Bush
Aug 15, 2009·European Journal of Mass Spectrometry·Gabi Drochioiu
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J A LooR D Smith

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Citations

Jun 19, 2012·Chemistry Central Journal·Dragos V NicaDespina-Maria Bordean
Mar 21, 2012·Chemistry Central Journal·Ducu Sandu Stef, Iosif Gergen
Mar 21, 2013·Journal of the American Society for Mass Spectrometry·Jonathan M DilgerDavid E Clemmer
Feb 1, 2015·Medical Hypotheses·Gabi DrochioiuManuela Murariu
Jul 12, 2013·European Journal of Mass Spectrometry·Gabi DrochioiuIonel Mangalagiu

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