Electrospray ionization with a pointed carbon fiber emitter

Analytical Chemistry
Jian LiuDaniel R Knapp

Abstract

A new type of electrospray ionization emitter employing a pointed carbon fiber has been developed for interfacing nanoliquid sampling techniques to mass spectrometry. The pointed carbon fiber protruding from an orifice with a surrounding hydrophobic surface confines a small Taylor cone at the tip, which generates a stable electrospray at the tip point. The small Taylor cone improves the electrospray efficiency thereby enhancing the detection limit. This emitter is rugged and able to generate stable electrospray over a wide range of flow rate, ESI voltage, and surface tension variation. Using a solution of angiotensin I, the carbon fiber emitter in 75-microm-i.d. fused-silica tubing was shown to give ion current comparable to that from a commercial 8 microm orifice nanospray emitter. Use of the emitter for ESI-MS/MS analysis of peptides was examined by infusing a mixture of cytochrome c and myoglobin tryptic digest peptides. Protein identification was demonstrated at the level of less than 1 fmol of the peptide consumed. The use of the carbon fiber emitter for interfacing monolithic capillary HPLC to MS was also demonstrated.

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Citations

Dec 15, 2012·Journal of the American Society for Mass Spectrometry·Bin HuZhong-Ping Yao
May 30, 2009·Mass Spectrometry Reviews·Graham T T GibsonRichard D Oleschuk
Aug 20, 2016·Rapid Communications in Mass Spectrometry : RCM·Di-Qiong JinQun Fang
Sep 9, 2016·Analytical Chemistry·Meng-Xi WuYin-Long Guo
Aug 24, 2006·Electrophoresis·Kyung Won RoDaniel R Knapp
Jun 5, 2007·Rapid Communications in Mass Spectrometry : RCM·Yong Seok Choi, Troy D Wood
Mar 22, 2008·Rapid Communications in Mass Spectrometry : RCM·Yong Seok Choi, Troy D Wood
Nov 23, 2019·Rapid Communications in Mass Spectrometry : RCM·Jiadi LinXiwei Wang
Aug 26, 2020·Electrophoresis·Anna TycovaFrantisek Foret
Sep 1, 2009·Analytical Chemistry·Shuqin SuRichard D Oleschuk

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