Electrospray mass spectrometry of alpha and beta chains of selected hemoglobins and their TNBA and TNB conjugates

Bioconjugate Chemistry
M Adamczyk, J C Gebler

Abstract

The molecular weights of alpha and beta hemoglobin chains from 15 different vertebrate animal sources and 2 common human variants were determined by electrospray mass spectrometry and compared to the calculated masses based on published amino acid sequences. Conjugates were prepared for 14 of the globins using 2 traditional colorimetric derivatizing reagents, 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and trinitrobenzenesulfonic acid (TNBS), and the mass of each conjugate was determined by mass spectrometry.

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Citations

Sep 9, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Natali Bauer, Andreas Moritz
Jun 9, 2000·Rapid Communications in Mass Spectrometry : RCM·M AdamczykJ Wu

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