Electrospun fiber surface nanotopography influences astrocyte-mediated neurite outgrowth

Biomedical Materials
Christopher D L JohnsonRyan J Gilbert

Abstract

Aligned, electrospun fiber scaffolds provide topographical guidance for regenerating neurons and glia after central nervous system injury. To date, no study has explored how fiber surface nanotopography affects astrocyte response to fibrous scaffolds. Astrocytes play important roles in the glial scar, the blood brain barrier, and in maintaining homeostasis in the central nervous system. In this study, electrospun poly L-lactic acid fibers were engineered with smooth, pitted, or divoted surface nanotopography. Cortical or spinal cord primary rat astrocytes were cultured on the surfaces for either 1 or 3 d to examine the astrocyte response over time. The results showed that cortical astrocytes were significantly shorter and broader on the pitted and divoted fibers compared to those on smooth fibers. However, spinal cord astrocyte morphology was not significantly altered by the surface features. These findings indicate that astrocytes from unique anatomical locations respond differently to the presence of nanotopography. Western blot results show that the differences in morphology were not associated with significant changes in glial fibrillary acidicprotein (GFAP) or vinculin in either astrocyte population, suggesting that surfac...Continue Reading

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Citations

Jun 5, 2018·Biomedical Materials·Tobias Führmann, Molly S Shoichet
Dec 22, 2020·Journal of Neuroscience Methods·Laura HonkamäkiSusanna Narkilahti
Apr 4, 2021·International Journal of Molecular Sciences·Hongyun XuanHuihua Yuan
Apr 15, 2020·ACS Applied Materials & Interfaces·Anne-Sophie MertgenMarkus Rottmar
May 8, 2019·ACS Applied Bio Materials·Christopher D L JohnsonRyan J Gilbert
Jan 19, 2021·ACS Biomaterials Science & Engineering·Alexis M ZiembaR Helen Zha

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