Abstract
Synthetic grafts comprised of a porous scaffold in the size and shape of the natural tracheobronchial tree, and autologous stem cells have shown promise in the ability to restore the structure and function of a severely damaged airway system. For this specific application, the selected scaffold material should be biocompatible, elicit limited cytotoxicity, and exhibit sufficient mechanical properties. In this research, we developed composite nanofibers of polycaprolactone (PCL) and depolymerized chitosan using the electrospinning technique and assessed the properties of the fibers for its potential use as a scaffold for regenerating tracheal tissue. Water-soluble depolymerized chitosan solution was first prepared and mixed with polycaprolactone solution making it suitable for electrospinning. Morphology and chemical structure analysis were performed to confirm the structure and composition of the fibers. Mechanical testing of nanofibers demonstrated both elastic and ductile properties depending on the ratio of PCL to chitosan. To assess biological potential, porcine tracheobronchial epithelial (PTBE) cells were seeded on the nanofibers with composition ratios of PCL/chitosan: 100/0, 90/10, 80/20, and 70/30. Transwell inserts we...Continue Reading
References
Dec 26, 2001·Tissue Engineering·S YangC K Chua
Dec 12, 2003·The Annals of Thoracic Surgery·Koji KojimaCharles A Vacanti
Apr 15, 2004·Journal of Colloid and Interface Science·Tamara ElzeinPhilippe Dumas
Aug 4, 2004·International Journal of Pharmaceutics·Shirui MaoThomas Kissel
May 12, 2005·Biomaterials·Narayan BhattaraiMiqin Zhang
Apr 11, 2008·Biomaterials·Jin San ChoiJames J Yoo
May 6, 2008·Tissue Engineering. Part C, Methods·Chen-Huan LinShan-Hui Hsu
Sep 23, 2008·Expert Review of Medical Devices·Volker StegerThorsten Walles
Jun 2, 2009·The Journal of Surgical Research·Yukihiro TatekawaMichio Kaneko
Nov 28, 2009·Seminars in Thoracic and Cardiovascular Surgery·Seth Kligerman, Amita Sharma
Jul 9, 2010·Revue des maladies respiratoires·E MartinodA Carpentier
Nov 6, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Bedilu A AlloKibret Mequanint
Nov 16, 2010·Carbohydrate Research·Ching Ting TsaoKuo Huang Hsieh
Nov 26, 2010·Interactive Cardiovascular and Thoracic Surgery·Hiroshi NaitoShigeki Taniguchi
Feb 8, 2011·Advanced Drug Delivery Reviews·Thorsten Walles
May 20, 2011·Annals of Biomedical Engineering·Lindsey M OttMichael S Detamore
Sep 25, 2012·Biomaterials·Bu-Kyu LeeJames J Yoo
Oct 10, 2012·Carbohydrate Polymers·Lien Van der SchuerenKaren De Clerck
Sep 26, 2013·European Journal of Medical Research·Emmanuel MartinodUNKNOWN FREnch Group for Airway Transplantation (FREGAT)
Apr 24, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Angela EdwardsNarayan Bhattarai
Aug 15, 2014·Nature Protocols·Philipp JungebluthPaolo Macchiarini
Sep 7, 2014·Acta Biomaterialia·Shih-Hsien ChenJyh-Ping Chen
Jan 13, 2015·Journal of Materials Science. Materials in Medicine·Tilak PrasadP R Anil Kumar
Citations
Apr 12, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Christopher JohnsonRohan A Shirwaiker
May 10, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Minghuan LiuYun-Ze Long
Dec 16, 2017·Materials·Saeid KargozarFrancesco Baino
Oct 21, 2017·Journal of Biomaterials Science. Polymer Edition·Elbay MalikmammadovNesrin Hasirci
May 3, 2018·Regenerative Medicine·Zhe ZhouMujun Lu
Jul 20, 2019·Journal of Biomaterials Science. Polymer Edition·Huilin YeYi Man
Sep 26, 2020·The Annals of Otology, Rhinology, and Laryngology·Ashley HeilingoetterTendy Chiang
May 14, 2017·Journal of Materials Science. Materials in Medicine·Lihua YinZhanhai Yu
Jan 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tong WuXiumei Mo
Dec 20, 2018·Journal of Cellular Physiology·Bharath Kumar VelmuruganRathinasamy Baskaran
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Cian O'LearySally-Ann Cryan
Jun 2, 2018·Stem Cells International·Adegbenro Omotuyi John FakoyaJoshua Yusuf
Nov 3, 2020·Nanoscale·Sheikh SaudiNarayan Bhattarai
Nov 29, 2020·Chemosphere·Preeti PalBrijesh Kumar Yadav
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Alexander J BoysRoisin M Owens
Mar 6, 2021·Biomaterials Science·Himadri Shekhar RoyDeepa Ghosh
May 10, 2021·International Journal of Biological Macromolecules·Parvaneh Ghaderi Shikhi-Abadi, Mohammad Irani
Apr 20, 2019·International Journal of Biological Macromolecules·Sruthi RanganathanNagarajan Selvamurugan
Jun 3, 2021·Progress in Biomaterials·B SowmyaP K Panda