Electrospun poly(D/L-lactide-co-L-lactide) hybrid matrix: a novel scaffold material for soft tissue engineering.

Journal of Materials Science. Materials in Medicine
Petra J KlugerKatja Schenke-Layland

Abstract

Electrospinning is a long-known polymer processing technique that has received more interest and attention in recent years due to its versatility and potential use in the field of biomedical research. The fabrication of three-dimensional (3D) electrospun matrices for drug delivery and tissue engineering is of particular interest. In the present study, we identified optimal conditions to generate novel electrospun polymeric scaffolds composed of poly-D/L-lactide and poly-L-lactide in the ratio 50:50. Scanning electron microscopic analyses revealed that the generated poly(D/L-lactide-co-L-lactide) electrospun hybrid microfibers possessed a unique porous high surface area mimicking native extracellular matrix (ECM). To assess cytocompatibility, we isolated dermal fibroblasts from human skin biopsies. After 5 days of in vitro culture, the fibroblasts adhered, migrated and proliferated on the newly created 3D scaffolds. Our data demonstrate the applicability of electrospun poly(D/L-lactide-co-L-lactide) scaffolds to serve as substrates for regenerative medicine applications with special focus on skin tissue engineering.

References

May 14, 1993·Science·R Langer, J P Vacanti
Oct 31, 2000·Biomaterials·J C Middleton, A J Tipton
Apr 12, 2002·Journal of Biomedical Materials Research·Wan-Ju LiFrank K Ko
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Sibylle GradMauro Alini
Mar 17, 2004·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Leena YlikontiolaNureddin Ashammakhi
Mar 2, 2005·Tissue Engineering·Zuwei MaSeeram Ramakrishna
Dec 20, 2005·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Jehad Al-SukhunRisto Kontio
Apr 4, 2006·Tissue Engineering·Ramalingam Murugan, Seeram Ramakrishna
Dec 16, 2006·Current Pharmaceutical Design·S Y ChewK W Leong
Sep 11, 2007·Advanced Drug Delivery Reviews·Sangmyung Rhee, Frederick Grinnell
Nov 30, 2007·Advanced Drug Delivery Reviews·Peter X Ma
Feb 19, 2008·Biomaterials·Travis J Sill, Horst A von Recum
Apr 12, 2008·Biomaterials·Sepideh Heydarkhan-HagvallWilliam R MacLellan
Apr 22, 2008·Trends in Biotechnology·Vladimir MironovRoger R Markwald
Jan 1, 2007·Recent Patents on Nanotechnology·Michelle NgiamCasey K Chan
Dec 17, 2008·Journal of Bodywork and Movement Therapies·Frederick Grinnell
Apr 4, 2009·Journal of Biophotonics·Katja Schenke-Layland
Feb 6, 2010·Expert Opinion on Biological Therapy·Richard P ViscontiVladimir Mironov
Aug 18, 2010·Macromolecular Bioscience·Miriam VottelerKatja Schenke-Layland

❮ Previous
Next ❯

Citations

Jun 26, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·Tina RuedigerBernd W Sigusch
Jan 10, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·Markus ReiseBernd W Sigusch
Jun 7, 2011·Acta Biomaterialia·Francesca IntranuovoMorgan R Alexander
Mar 15, 2011·Advanced Drug Delivery Reviews·Esther C NovoselPetra J Kluger
Mar 29, 2012·Journal of Cellular and Molecular Medicine·Patcharakamon NooeaidAldo R Boccaccini
May 15, 2016·Biointerphases·Michael EggerAlexander Southan
Jun 6, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Dennis SchkarpetkinBernd W Sigusch
Sep 29, 2017·Journal of Materials Science. Materials in Medicine·Dariush SemnaniMehrnoosh Maherolnaghsh
Feb 28, 2015·Advanced Healthcare Materials·Mohammad NorouziMasoud Soleimani

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
fluorescence microscopy

Software Mentioned

Adobe Photoshop
Pro Plus
Image

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.