Electrospun poly(methyl methacrylate)/polyaniline fibres as a support for laccase immobilisation and use in dye decolourisation

Environmental Research
Katarzyna JankowskaTeofil Jesionowski

Abstract

Novel electrospun poly(methyl methacrylate)/polyaniline electrospun fibres were produced, characterised, modified, and used as a support for laccase immobilisation by two methods: adsorption and covalent binding. Effective deposition of laccase by both methods was confirmed by FTIR and CLSM results. Nevertheless, the main objective of the study was to select the most favourable immobilisation conditions and prepare heterogeneous biocatalysts with the best possible catalytic properties. The highest relative activity of enzymes immobilised by adsorption and covalent binding were obtained after 1 h of immobilisation using laccase solution at a concentration of 1 mg/mL, at pH 5 and 25 °C. It was found that the immobilised enzymes, which were present in amounts of 110 mg/g and 185 mg/g for systems with adsorbed and covalently bonded laccase respectively, exhibited slightly lower substrate affinity, and in consequence also a lower maximum reaction rate, than the free enzyme. The stability of laccase improved significantly upon immobilisation: both heterogeneous biocatalysts retained over 80% relative activity even after 10 repeated catalytic cycles and 30 days of storage. The obtained systems were used for decolourisation of Remazol ...Continue Reading

Citations

Oct 27, 2020·Frontiers in Microbiology·Sherine Ahmed Gamal Zakaria MorsyFairolniza Mohd Shariff
Jan 14, 2021·Biotechnology & Genetic Engineering Reviews·Dajun RenXiaoqing Zhang
Apr 6, 2021·Critical Reviews in Biotechnology·Kai SunQingguo Huang

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