Electrospun Structures Made of a Hydrolyzed Keratin-Based Biomaterial for Development of in vitro Tissue Models

Frontiers in Bioengineering and Biotechnology
Gabriele Maria FortunatoGiovanni Vozzi

Abstract

The aim of this study is the analysis and characterization of a hydrolyzed keratin-based biomaterial and its processing using electrospinning technology to develop in vitro tissue models. This biomaterial, extracted from poultry feathers, was mixed with type A porcine gelatin and cross-linked with γ-glycidyloxy-propyl-trimethoxy-silane (GPTMS) to be casted initially in the form of film and characterized in terms of swelling, contact angle, mechanical properties, and surface charge density. After these chemical-physical characterizations, electrospun nanofibers structures were manufactured and their mechanical properties were evaluated. Finally, cell response was analyzed by testing the efficacy of keratin-based structures in sustaining cell vitality and proliferation over 4 days of human epithelial, rat neuronal and human primary skin fibroblast cells.

References

Aug 1, 1996·Journal of Biomedical Materials Research·K YamauchiY Konishi
Apr 3, 2004·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Fouad KhatyrJean-Michel Lagarde
Jul 28, 2004·Biomaterials·F J O'BrienL J Gibson
Sep 25, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yanzhong ZhangZheng-Ming Huang
Jun 20, 2007·Journal of Materials Science. Materials in Medicine·Ju-Ha SongHae-Won Kim
Jun 16, 2011·International Journal of Molecular Sciences·Tsung-Lin Yang
Jun 18, 2011·Journal of Biomedical Materials Research. Part a·Justin M SaulMark Van Dyke
Aug 31, 2011·Pakistan Journal of Biological Sciences : PJBS·Srihanam Prasong, Tessanan Wasan
Nov 22, 2011·Journal of the Mechanical Behavior of Biomedical Materials·Aisling Ní AnnaidhMélanie Otténio
Jan 31, 2012·Cell and Tissue Research·Shuai WangKee Woei Ng
Jun 28, 2013·Journal of Biomedical Nanotechnology·P R SreerekhaK P Chennazhi
Jul 20, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuichi Tsuda, Yoshihiro Nomura
Oct 8, 2013·International Journal of Biological Macromolecules·S SaravananN Selvamurugan
Jan 28, 2015·Journal of Colloid and Interface Science·Tripta KamraLei Ye
Feb 8, 2016·Colloids and Surfaces. B, Biointerfaces·Hsun-Yun ChangJing-Jong Shyue
Nov 16, 2017·Trends in Biotechnology·Lorenzo MoroniGiovanni Vozzi

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Citations

Apr 24, 2020·Bioactive Materials·Sandleen FerozGeorge Dias
May 14, 2021·International Journal of Biological Macromolecules·Logesh Kumar SellappanSwathy Manoharan
Jun 27, 2021·Macromolecular Bioscience·Anna LapomardaGiovanni Vozzi
Feb 11, 2020·Biomacromolecules·Jukka SeppäläAndreas Lendlein

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Methods Mentioned

BETA
bioprinting
scanning electron microscopy
confocal microscopy

Software Mentioned

ImageJ
Matlab®
Matlab
CAM

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