PMID: 9440872Jan 24, 1998Paper

Electrostatic endothelial cell seeding technique for small-diameter (<6 mm) vascular prostheses: feasibility testing

Cell Transplantation
Gary L Bowlin, Stanley E Rittgers

Abstract

Multiple studies have indicated the importance of surface charge in the adhesion of multiple cardiovascular cell lines including platelets and endothelial cells on the substrate materials (1,4,7-10,12-15). It is the purpose of this article to report a feasibility study conducted using an electrostatic endothelial cell seeding technique. The feasibility study was conducted using human umbilical vein endothelial cells (HUVEC), a static pool apparatus, a voltage source, and a parallel plate capacitor. The HUVEC concentration and seeding times were constant at 560,000 HUVEC/ml and 30 min, respectively. Scanning electron microscopy examination of the endothelial cell adhesion indicated that an induced temporary positive surface charge on e-PTFE graft material enhances the number and the maturation (flattening) of HUVECs adhered. The results indicated that the total number of endothelial cells adhered (70.9 mm2) was increased from 9198 +/- 1194 HUVECs on the control (no induced surface charge) e-PTFE to 22,482 +/- 4814 HUVECs (2.4 x control) on the maximum induced positive surface charge. The total number of cells in the flattened phase of adhesion increased from 837 +/- 275 to 6785 +/- 1012 HUVECs (8.1x) under identical conditions. ...Continue Reading

Citations

Jan 4, 2005·Blood Purification·Prabir Roy-ChaudhurySue C Heffelfinger
Oct 3, 2007·Regenerative Medicine·Rei OgawaHike Hyakusoku
Jan 28, 2005·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Peter S McFetridgeJulian B Chaudhuri
Dec 17, 2008·Tissue Engineering. Part C, Methods·Feng ZhaoTeng Ma
May 7, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Qian ZhouJun Li
Aug 9, 2005·Cardiology Clinics·Prabir Roy-ChaudhurySue C Heffelfinger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.