Electrostatic interactions across a beta-sheet

Biochemistry
C A Blasie, J M Berg

Abstract

The free energy consequences of electrostatic interactions across a model beta-sheet have been probed using a consensus zinc finger peptide. Relative folding free energies have been deduced from coupled peptide folding/metal binding reactions. The energies of the electrostatic interactions have been isolated via double mutant cycles performed at a series of NaCl concentrations. The observed favorable free energies associated with potential ion pairs are modest, less than 0.5 kcal/mol. Unfavorable interactions due to like-charge pairs of similar magnitude were also observed. The largest effects, both favorable and unfavorable, involved interactions with aspartic acid. These observations are consistent with electrostatic interactions occurring without contact ion pair formation with the larger interaction energies involving aspartic acid being due to the relative lack of flexibility of its side chain.

References

Dec 20, 1990·Journal of Molecular Biology·A HorovitzA R Fersht
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A D FrankelC O Pabo

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Citations

Mar 5, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Wenpeng KouGregg R Dieckmann
Sep 7, 2002·Journal of Molecular Biology·Mark D DistefanoAndrea G Cochran
Mar 29, 2003·Journal of Molecular Biology·George I MakhatadzeSusan T Thomas
Aug 17, 1999·Current Opinion in Structural Biology·E LacroixL Serrano
Nov 26, 2010·Journal of the American Chemical Society·Olivier Sénèque, Jean-Marc Latour
Feb 16, 2002·Protein Science : a Publication of the Protein Society·Kirsten S LassilaStephen L Mayo
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Kelly K LeeBertrand García-Moreno E
Jun 23, 2001·Protein Science : a Publication of the Protein Society·M Ramírez-AlvaradoL Serrano
Oct 6, 2015·Biomolecules·Junji IwaharaLevani Zandarashvili
May 30, 2014·ACS Chemical Biology·Andrew M Watkins, Paramjit S Arora
Mar 5, 2016·Molecular BioSystems·Angelique N BesoldDavid P Goldberg
Sep 20, 2015·Archives of Biochemistry and Biophysics·Rishu JainRajesh Kumar
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Sarah E Kiehna, Marcey L Waters
Sep 22, 2010·Chemistry : a European Journal·Muhammad Irfan AshiqJeremy D Kilburn
Jan 23, 2003·Protein Science : a Publication of the Protein Society·Tamas BlandlNicholas J Skelton
May 5, 1998·Folding & Design·M StruthersB Imperiali
Feb 1, 2007·Chemical Society Reviews·Scott L Cockroft, Christopher A Hunter
Sep 10, 2008·The Journal of Biological Chemistry·Meng GeXian-Ming Pan
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cheng-Hsin HuangRichard P Cheng
Nov 17, 2001·Chemical Reviews·J VenkatramanP Balaram
Feb 12, 2004·Chemical Reviews·Derek JantzJeremy M Berg
Dec 8, 2004·Inorganic Chemistry·Karen R ThickmanJeremy M Berg
Sep 25, 2021·Protein Science : a Publication of the Protein Society·Suhas H BhateRanabir Das
Mar 24, 2005·Journal of the American Chemical Society·Scott T PhillipsPaul A Bartlett

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