Electrostatic interactions between capsid and scaffolding proteins mediate the structural polymorphism of a double-stranded RNA virus.

The Journal of Biological Chemistry
Irene SaugarJosé R Castón

Abstract

Capsid proteins that adopt distinct conformations constitute a paradigm of the structural polymorphism of macromolecular assemblies. We show the molecular basis of the flexibility mechanism of VP2, the capsid protein of the double-stranded RNA virus infectious bursal disease virus. The initial assembly, a procapsid-like structure, is built by the protein precursor pVP2 and requires VP3, the other infectious bursal disease virus major structural protein, which acts as a scaffold. The pVP2 C-terminal region, which is proteolyzed during virus maturation, contains an amphipathic alpha-helix that acts as a molecular switch. In the absence of VP3, efficient virus-like particle assembly occurs when the structural unit is a VP2-based chimeric protein with an N-terminal-fused His(6) tag. The His tag has a positively charged N terminus and a negatively charged C terminus, both important for virion-like structure assembly. The charge distributions of the VP3 C terminus and His tag are similar. We tested whether the His tag emulates the role of VP3 and found that the presence of a VP3 C-terminal peptide in VP2-based chimeric proteins resulted in the assembly of virus-like particles. We analyzed the electrostatic interactions between these ...Continue Reading

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Citations

May 24, 2012·The Journal of Biological Chemistry·Nerea IrigoyenJosé F Rodríguez
Nov 27, 2015·Virology·Yung-Yi C MosleyTsang Long Lin
Oct 31, 2017·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Elena PascualJosé R Castón
Mar 16, 2018·Journal of Virology·María Cecilia GimenezLaura Ruth Delgui
Jul 20, 2018·Journal of Virology·Carlos P MataJosé R Castón
Oct 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Siyu LiRoya Zandi
Jan 22, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Pedro BuzónWouter H Roos
Oct 21, 2016·Chemistry : a European Journal·Jingfang LiWen Li
Nov 13, 2012·Archives of Biochemistry and Biophysics·Mauricio G Mateu

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