Electrostatic melting in a single-molecule field-effect transistor with applications in genomic identification

Nature Communications
Sefi VernickKenneth L Shepard

Abstract

The study of biomolecular interactions at the single-molecule level holds great potential for both basic science and biotechnology applications. Single-molecule studies often rely on fluorescence-based reporting, with signal levels limited by photon emission from single optical reporters. The point-functionalized carbon nanotube transistor, known as the single-molecule field-effect transistor, is a bioelectronics alternative based on intrinsic molecular charge that offers significantly higher signal levels for detection. Such devices are effective for characterizing DNA hybridization kinetics and thermodynamics and enabling emerging applications in genomic identification. In this work, we show that hybridization kinetics can be directly controlled by electrostatic bias applied between the device and the surrounding electrolyte. We perform the first single-molecule experiments demonstrating the use of electrostatics to control molecular binding. Using bias as a proxy for temperature, we demonstrate the feasibility of detecting various concentrations of 20-nt target sequences from the Ebolavirus nucleoprotein gene in a constant-temperature environment.

References

Oct 28, 1974·Biophysics of Structure and Mechanism·P Valenta, H W Nürnberg
Dec 1, 1995·Biophysical Journal·V ChanS E McKenzie
Jul 20, 2002·Current Opinion in Structural Biology·Mark C Williams, Ioulia Rouzina
May 22, 2003·Analytical Biochemistry·David EricksonUlrich J Krull
Jul 15, 2003·Physical Review Letters·Alexis F Sauer-BudgeDaniel Branton
Jul 9, 2004·Biophysical Journal·Jonathan NakaneAndre Marziali
Sep 7, 2004·Biophysical Journal·Jérôme MathéAmit Meller
Nov 4, 2005·Analytical Biochemistry·Martin MehlmannKathy L Rowlen
Jun 13, 2006·Biophysical Journal·Sean A McKinneyTaekjip Ha
Aug 16, 2006·Physical Review Letters·Jaan MannikPhilip G Collins
Aug 31, 2006·Biochemistry·Anna TikhomirovaTigran V Chalikian
Jan 6, 2007·Science·Brett R GoldsmithPhilip G Collins
Jan 15, 2009·Chemistry : a European Journal·Grégory SchmidtPascale Chenevier
Nov 10, 2009·Journal of the American Chemical Society·David Yu Zhang, Erik Winfree
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·David GreshamDavid Botstein
Jun 21, 2011·Genes & Development·Ignacio Tinoco, Ruben L Gonzalez
Aug 3, 2011·Nano Letters·Sebastian SorgenfreiKenneth L Shepard
Dec 24, 2011·Bioelectrochemistry·Robert P JohnsonPhilip N Bartlett
Jan 24, 2012·Science·Yongki ChoiPhilip G Collins
Aug 13, 2013·Nucleic Acids Research·Thomas E OuldridgeArd A Louis
Jan 15, 2014·Biophysical Journal·Erik D HolmstromDavid J Nesbitt
May 9, 2014·The Journal of Chemical Physics·Karthikeyan Marimuthu, Raj Chakrabarti
May 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicholas F DupuisDavid J Nesbitt
Feb 5, 2015·Nanotechnology·Calin Plesa, Cees Dekker
Jul 7, 2015·Bioelectrochemistry·Evanthia PapadopoulouPhilip N Bartlett
Jun 9, 2016·Nano Letters·Delphine BouillyColin Nuckolls

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Citations

Mar 21, 2020·Biosensors·Jonathan D Bohbot, Sefi Vernick
Jan 7, 2021·Nanoscale·Shima Ghasemi, Kasper Moth-Poulsen
Mar 9, 2021·ACS Applied Materials & Interfaces·Ting WuAlison J Downard
Sep 19, 2018·Chemical Reviews·Vera SchroederTimothy M Swager

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Methods Mentioned

BETA
chip
PCR
AFM
chips

Software Mentioned

smFET
HaMMy
MATLAB

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