Electrotransformation of Lactobacillus plantarum using linearized plasmid DNA

Letters in Applied Microbiology
J K ThompsonM A Collins

Abstract

Evidence is presented that linearized plasmid DNA is capable of electrotransforming Lactobacillus plantarum at a frequency 500-fold lower than with the covalently closed circular molecule. When the linearized plasmid was religated prior to transformation the transformation efficiency was < 10-fold higher, suggesting that open circular plasmid was only slightly more efficient in the transformation of Lact. plantarum than linear DNA. This observation has implications for direct cloning into this species since the high background transformation frequency produced by the linear DNA could potentially obscure the recovery of clones. Nevertheless, using positive selection for enhanced chloramphenicol resistance, cloned fragments of Lact. helveticus DNA were obtained using the shuttle vector pGKV110.

References

Apr 1, 1991·Applied and Environmental Microbiology·G M DunnyD J LeBlanc
Nov 25, 1989·Nucleic Acids Research·C M CollisH R Drew
Aug 1, 1989·Applied and Environmental Microbiology·E E BatesS P Mann
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Dec 15, 1994·FEMS Microbiology Letters·D PridmoreB Mollet
Jan 1, 1993·Antonie van Leeuwenhoek·P H Pouwels, R J Leer

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Citations

May 4, 2021·Journal of the Science of Food and Agriculture·Ling-Hui KongLian-Zhong Ai
Oct 27, 2021·World Journal of Microbiology & Biotechnology·Qi LiSheng Yang

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