Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

Applied and Environmental Microbiology
Pascale SerrorEmmanuelle Maguin

Abstract

We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 10(4) transformants per microg of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on phage integrative elements into the L. delbrueckii subsp. bulgaricus chromosome. These vectors provide a good basis for developing molecular tools for L. delbrueckii and open the field of genetic studies in L. delbrueckii.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1992·Journal of Bacteriology·E MaguinA Gruss
Jun 1, 1990·Agricultural and Biological Chemistry·H HashibaK Aoyama
May 1, 1989·The Journal of Applied Bacteriology·R Fuller
Jan 1, 1985·Gene·L JannièreS Dusko Ehrlich
Dec 1, 1988·Applied and Environmental Microbiology·B BoizetB M Chassy
Jan 1, 1995·Methods in Molecular Biology·T W AukrustI F Nes
Feb 7, 2001·Applied and Environmental Microbiology·J P JoreP H Pouwels
Jun 1, 1975·Applied Microbiology·B E Terzaghi, W E Sandine
May 1, 1996·Microbiology·Françoise BerthierFrançoise Morel-Deville

Citations

Aug 19, 2007·Applied Microbiology and Biotechnology·Je-Ruei LiuKuo-Joan Cheng
Oct 14, 2003·FEMS Microbiology Letters·Pascale SerrorEmmanuelle Maguin
May 23, 2012·Applied and Environmental Microbiology·Hélène Licandro-SerautPascale Serror
Jul 6, 2006·Applied and Environmental Microbiology·Sigrid C J De KeersmaeckerPascal Hols
Jun 6, 2006·Applied and Environmental Microbiology·Jan-Peter van PijkerenPaul W O'Toole
Jul 9, 2004·Applied and Environmental Microbiology·Elena BorodinaJ Colin Murrell
Nov 5, 2005·Applied and Environmental Microbiology·Je-Ruei LiuXin Zhao
Sep 12, 2012·Journal of Molecular Microbiology and Biotechnology·Wang LiYulin Chen
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·M van de GuchteEmmanuelle Maguin
Feb 18, 2016·Journal of Biotechnology·Ruangurai PromchaiPlearnpis Luxananil
Jul 23, 2014·International Journal of Molecular Sciences·Lei WangYulin Chen
Oct 5, 2015·Applied Microbiology and Biotechnology·Yixing ZhangAkihiko Kondo
Sep 3, 2010·Journal of Microbiological Methods·Maria Mercedes PalominoSandra M Ruzal
Jan 23, 2009·International Journal of Food Microbiology·Plearnpis LuxananilRuud Valyasevi
Feb 21, 2007·Journal of Applied Microbiology·J LöfblomH Wernérus
Mar 1, 2006·Journal of Microbiological Methods·Diego RomeroOscar P Kuipers
Aug 3, 2014·Journal of Microbiological Methods·José M LandeteMargarita Medina
Oct 11, 2012·International Journal of Biological Macromolecules·Wacim BejarHichem Chouayekh
Aug 2, 2003·FEMS Microbiology Letters·Soo Jin JangDae Kyun Chung
Jan 31, 2006·Veterinary Microbiology·F A DorellaA Miyoshi
Oct 25, 2016·PloS One·Elham AslankoohiKevin J Verstrepen

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