Electrotrophic activity and electrosynthetic acetate production by Desulfobacterium autotrophicum HRM2

Bioelectrochemistry
Zehra ZaybakJohn M Pisciotta

Abstract

Electroautotrophic microorganisms accept electrons from a cathode as source of reducing equivalents to drive CO2 fixation by poorly understood mechanisms. Acetogenic bacteria were the first group found to possess the capability for electroautotrophic metabolism in pure culture with associated electrosynthesis of acetate as primary metabolite. Identification of additional electrotrophic species can contribute to our understanding of this unusual form of metabolism. Here, bioelectrochemical techniques, chemical analysis and microscopy were used to determine electrotrophic metabolism of Desulfobacterium autotrophicum HRM2. Chronoamperometry showed increasing current uptake over 21 days of incubation in duplicate bioelectrochemical system sets. Linear sweep voltammetry indicated peak current uptake at -243 mV. High performance liquid chromatography (HPLC) analysis quantified acetate accumulation in anaerobic minimal media containing inorganic carbon as sole carbon source, consistent with electrosynthesis. Scanning electron microscopy and live/dead staining by epifluorescence microscopy analysis indicated viable 1-2 μm cells after 76 days of cultivation under electroautotrophic conditions. The genome of Db. autotrophicum HRM2 is ful...Continue Reading

Citations

Aug 25, 2019·The ISME Journal·Paola Andrea PalaciosAmelia-Elena Rotaru
Jun 9, 2020·Frontiers in Bioengineering and Biotechnology·Valeria AgostinoMiriam A Rosenbaum
Sep 16, 2020·Journal of Industrial Microbiology & Biotechnology·Dinesh GuptaArpita Bose

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