Elegant chemistry to directly anchor intact saccharides on solid surfaces used for the fabrication of bioactivity-conserved saccharide microarrays

Bioconjugate Chemistry
Kai Liang, Yi Chen

Abstract

An easy chemical strategy was proposed and used to establish a method for direct anchoring of intact saccharides on solid surfaces with well conserved bioaffinity. The anchoring was achieved by temperature-modulated stepwise reactions with cyanuric chloride as a key linker, and was successfully applied to the fabrication of saccharide chips. To demonstrate, 15 intact reducing and nonreducing saccharides with various molecular sizes were dotted on a cyanuric-chloride-modified chip (1.0 × 1.0 cm(2)) and made to react with lectins. As expected, the anchored saccharides were capable of recognizing their target lectins, and more exciting were the perfect conservation of the specific recognizing ability of the anchored monosaccharides such as mannose, glucose, and even fructose (interacting only weakly with concanavalin A). This conservation was ascribed to the maintenance of the original structure (especially the anomeric configuration) of saccharides after immobilization and to the allowance of the anchored saccharides to rotate with and/or on the scaffold of cyanuric chloride, which makes them easily adapt to the recognition-preferred spatial position. The expected linkage of saccharides on cyanuric chloride and the maintenance of...Continue Reading

References

Jan 1, 1977·Journal of Immunological Methods·H ElwingO Ouchterlony
Sep 1, 1990·Bio/technology·W C Skarnes
Jan 1, 1971·Experimental Cell Research·W Bernhard, S Avrameas
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J HolmgrenO Strannegård
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·D N Moothoo, J H Naismith
Sep 2, 2000·The Journal of Organic Chemistry·Y HatanakaP Jong-Jip
Nov 14, 2000·Biopolymers·Z J Kamiński
Jan 17, 2002·Bioconjugate Chemistry·Paul H LeeMark A Reynolds
Feb 19, 2002·Chemical Reviews·Halina Lis, Nathan Sharon
May 2, 2002·Chemistry & Biology·Benjamin T Houseman, Milan Mrksich
Apr 15, 2004·Journal of the American Chemical Society·Sungjin ParkInjae Shin
Jul 15, 2004·Journal of the American Chemical Society·Marian C BryanChi-Huey Wong
Sep 3, 2004·Glycobiology·S AngeloniN Sprenger
Nov 9, 2004·Journal of Combinatorial Chemistry·Mahesh UttamchandaniYoung-Tae Chang
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ola BlixtJames C Paulson
Jan 7, 2005·Chemistry : a European Journal·Injae ShinMyung-ryul Lee
Sep 22, 2005·Journal of the American Chemical Society·Kwang-Seuk KoNicola L Pohl
Oct 28, 2005·Chembiochem : a European Journal of Chemical Biology·Joseph C ManimalaJeffrey C Gildersleeve
Nov 10, 2005·Genome Biology·Christopher T Campbell, Kevin J Yarema
Mar 2, 2006·Journal of the American Chemical Society·Jose L de PazPeter H Seeberger
Mar 8, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Gregory T CarrollJeffrey T Koberstein
Apr 19, 2006·Nature Chemical Biology·James C PaulsonBrian E Collins
Jun 15, 2006·Journal of the American Chemical Society·Sarah E TullyLinda C Hsieh-Wilson
Jul 5, 2007·Glycoconjugate Journal·Gregory T CarrollJeffrey T Koberstein
Apr 3, 2008·Angewandte Chemie·Lan Ban, Milan Mrksich
Jun 24, 2008·Chemical Society Reviews·Tim Horlacher, Peter H Seeberger
Jul 24, 2008·Chemical Society Reviews·Sungjin ParkInjae Shin
Aug 30, 2008·Analytical Chemistry·Johanna-Gabriela WalterThomas Scheper
Sep 20, 2008·Chemical Communications : Chem Comm·Sungjin ParkInjae Shin
Sep 20, 2008·Chemical Communications : Chem Comm·Nicolas LaurentSabine L Flitsch
Apr 16, 2009·Journal of Proteome Research·Oyindasola OyelaranJeffrey C Gildersleeve
Sep 5, 2009·ACS Chemical Biology·Lakshmi Krishnamoorthy, Lara K Mahal
Nov 26, 2009·Expert Review of Proteomics·Chi-Hui Liang, Chung-Yi Wu
Jun 30, 2010·Journal of the American Chemical Society·Yalong ZhangJeffrey C Gildersleeve

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Citations

Oct 12, 2015·Carbohydrate Research·Daniel Hernandez ArmadaChristopher W Cairo
Apr 15, 2016·Biomaterials Science·P Bojarová, V Křen
Sep 7, 2014·Analytical Methods : Advancing Methods and Applications·A Hushegyi, J Tkac
Nov 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Songzhi XieXiaohong Li
Dec 7, 2018·Journal of Carbohydrate Chemistry·Crystal L O'NeilAlexei V Demchenko
Sep 26, 2019·Nanoscale·Long ZhaoXiaohong Li
Mar 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Wei YangYi Chen

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