Elemental and isotopic imaging of biological samples using NanoSIMS

Methods in Molecular Biology
Matt R Kilburn, Peta L Clode

Abstract

With its low detection limits and the ability to analyze most of the elements in the periodic table, secondary ion mass spectrometry (SIMS) represents one of the most versatile in situ analytical techniques available, and recent developments have resulted in significant advantages for the use of imaging mass spectrometry in biological and biomedical research. Increases in spatial resolution and sensitivity allow detailed interrogation of samples at relevant scales and chemical concentrations. Advances in dynamic SIMS, specifically with the advent of NanoSIMS, now allow the tracking of stable isotopes within biological systems at subcellular length scales, while static SIMS combines subcellular imaging with molecular identification. In this chapter, we present an introduction to the SIMS technique, with particular reference to NanoSIMS, and discuss its application in biological and biomedical research.

Citations

May 23, 2015·Critical Reviews in Analytical Chemistry·Kamil JurowskiWojciech Piekoszewski
Feb 28, 2018·Nature Microbiology·Andrew R J CursonJonathan D Todd
Apr 2, 2020·International Journal of Molecular Sciences·Barbara WittTanja Schwerdtle
Nov 6, 2014·Biomolecular Concepts·Constanze Pinske, R Gary Sawers

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