Elemental bioimaging of thulium in mouse tissues by laser ablation-ICPMS as a complementary method to heteronuclear proton magnetic resonance imaging for cell tracking experiments

Analytical Chemistry
Olga ReifschneiderUwe Karst

Abstract

Due to the fact that cellular therapies are increasingly finding application in clinical trials and promise success by treatment of fatal diseases, monitoring strategies to investigate the delivery of the therapeutic cells to the target organs are getting more and more into the focus of modern in vivo imaging methods. In order to monitor the distribution of the respective cells, they can be labeled with lanthanide complexes such as thulium-1,4,7,10-tetraazacyclodoecane-α,α,α,α-tetramethyl-1,4,7,10-tetraacetic acid (Tm(DOTMA)). In this study, experiments on a mouse model with two different cell types, namely, tumor cells and macrophages labeled with Tm(DOTMA), were performed. The systemic distribution of Tm(DOTMA) of both cell types was investigated by means of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICPMS). Using the high resolution of 25 μm, distribution maps of Tm in different tissues such as tumor, liver, lung, and spleen as well as in explanted gel pellets were generated and the behavior of the labeled cells inside the tissue was investigated. Additionally, quantitative data were obtained using homemade matrix-matched standards based on egg yolk. Using this approach, limits of detection and quantific...Continue Reading

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Aug 13, 2013·Metallomics : Integrated Biometal Science·Olga ReifschneiderUwe Karst

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Citations

Sep 14, 2016·Analytica Chimica Acta·Ann-Christin NiehoffUwe Karst
Nov 11, 2019·Analytical and Bioanalytical Chemistry·Mauro Martinez, Matthieu Baudelet
Mar 23, 2017·Analytical and Bioanalytical Chemistry·Larissa MuellerNorbert Jakubowski
Nov 4, 2017·Contrast Media & Molecular Imaging·Ann-Christin NiehoffRebecca Hadrian
May 22, 2021·Chemical Reviews·Philip A DobleDavid Clases

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