Elemental composition of secretory granules in pancreatic islets of Langerhans

Biophysical Journal
M C FosterI Atwater

Abstract

We have characterized, by electron probe microanalysis, rapidly frozen cultured rat islets at the level of individual secretory granules. Elemental analysis of thin, dried cryosections showed that beta granules could be distinguished by high Zn, Ca, and S, whereas non-beta (mainly alpha) granules contained elevated P and Mg. Although a single granule type predominated in a particular cell, some rebel granules were found in A cells that had the compositional fingerprint of B cell granules. Zn, which was found in millimolar concentrations in B cell granules, was considered a marker for the insulin storage complex. The data indicate that non-B islet cells in the adult pancreas may produce insulin-containing organelles and that, when glucagon and insulin are coexpressed, these hormones are packaged in separate granules.

Citations

Apr 20, 2010·Pflügers Archiv : European journal of physiology·Thomas F J WagnerJohannes Oberwinkler
Jan 5, 2013·Nutrition Research Reviews·Fabrice Chimienti
Jul 7, 2009·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·M D YnsaF Watt
Jun 12, 2010·Journal of the American Chemical Society·Jeffrey R BrenderAyyalusamy Ramamoorthy
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Angelo J M LubagA Dean Sherry
Jul 5, 2012·The Journal of Biological Chemistry·David Moreno-SanchezRoberto Docampo
Oct 4, 2006·Journal of the Royal Society, Interface·Christopher C BroomellJ Herbert Waite
Oct 13, 2009·Diabetes, Obesity & Metabolism·N WijesekaraM B Wheeler
Aug 28, 2013·Endocrine·Yang V Li
Aug 14, 2001·Biochemical and Biophysical Research Communications·W J Qian, R T Kennedy
Dec 25, 2015·Journal of the American Chemical Society·Nadezhda NespovitayaRoland Riek
Mar 9, 2004·Analytical Biochemistry·Anne T MaghasiHoracio L Rodriguez Rilo
Aug 22, 2015·Current Diabetes Reports·William T MooreDongmin Liu
Mar 17, 2015·Endocrine·Kira G SlepchenkoYang V Li
Feb 14, 2012·European Journal of Pharmacology·Kalavakunda VijayaraghavanSorimuthu Pillai Subramanian
Jun 6, 2016·Archives of Biochemistry and Biophysics·Pauline Chabosseau, Guy A Rutter
May 27, 2016·Antioxidants & Redox Signaling·Philipp A Gerber, Guy A Rutter
Nov 18, 2015·Journal of Diabetes Research·Phuong Trang NguyenSteve Bourgault
Jul 28, 2016·Biophysical Journal·Zeno LavagninoDavid W Piston
Nov 20, 2015·Journal of Diabetes Research·Marianna Flora TomaselloGiuseppe Pappalardo
Oct 4, 2016·Biophysical Chemistry·Luiza C S ErthalLuís Maurício T R Lima
Nov 3, 2016·Biological & Pharmaceutical Bulletin·Khalil Al-AliYaser Abdel-Moemen El-Badry
Feb 12, 1998·The Journal of Biological Chemistry·C A AspinwallJ R Lakey
Mar 31, 2015·Chemical Science·Pablo Rivera-FuentesStephen J Lippard
Jan 15, 2005·Cell Transplantation·T B MurdochJ R T Lakey
Feb 9, 2018·International Journal of Molecular Sciences·Ayako Fukunaka, Yoshio Fujitani
Dec 18, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L G SøndergaardJ Rungby
Mar 10, 2017·Physiological Reviews·T D MüllerM H Tschöp
Mar 18, 2003·Nature Cell Biology·Hisamitsu IshiharaClaes B Wollheim
Mar 1, 1996·The Histochemical Journal·M StoltenbergG Danscher
Jan 13, 2006·The Journal of Biological Chemistry·Armen V GyulkhandanyanMichael B Wheeler
Feb 6, 2008·The Journal of Biological Chemistry·Armen V GyulkhandanyanMichael B Wheeler
Apr 14, 2019·The Journal of Biological Chemistry·Pamela L ToledoMario R Ermácora
May 24, 2011·The Journal of Biological Chemistry·Wei YangLin-Hua Jiang
Aug 6, 2011·Chemical Society Reviews·Alaina S DeTomaMi Hee Lim
May 3, 2013·Chemical Communications : Chem Comm·Nicolas P E Barry, Peter J Sadler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.