Elements proximal to and within the transmembrane domain mediate the organelle-to-organelle movement of bZIP28 under ER stress conditions

The Plant Journal : for Cell and Molecular Biology
Renu SrivastavaStephen H Howell

Abstract

Arabidopsis bZIP28, an ER membrane-associated transcription factor, is activated in response to conditions that induce ER stress-adverse environmental conditions or exposure to ER stress agents such as tunicamycin and dithiothreitol. Upon stress treatment, bZIP28 exits the ER and moves to the Golgi, where it is proteolytically processed, releasing its transcriptional component, which relocates to the nucleus. In this study, we tracked the movement of GFP-tagged bZIP28 in an effort to understand its mobilization from the ER and release from the Golgi. We identified a small region in bZIP28 that is rich in dibasic amino acids and proximal to the transmembrane domain required for its movement from the ER. In response to ER stress, bZIP28 showed enhanced interaction with Sar1 and Sec12, components of the COPII machinery. We demonstrated that the dibasic amino acid-rich region in bZIP28 is involved in the interaction with Sar1. Upon migration to the Golgi, bZIP28 is proteolytically processed by proteases S1P and S2P. We found a putative helix-breaking residue in the transmembrane domain of bZIP28 to be crucial for its processing and liberation from Golgi bodies. Thus, in response to stress, bZIP28 moves from organelle to organelle b...Continue Reading

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Citations

Feb 2, 2013·Plant Molecular Biology·Kyoko KudoFumio Takaiwa
Jan 22, 2013·Annual Review of Plant Biology·Stephen H Howell
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yonglun ZengLiwen Jiang
Jul 8, 2015·Current Opinion in Plant Biology·Cristina RubertiFederica Brandizzi
Jun 14, 2014·Frontiers in Plant Science·Brett WilliamsMartin B Dickman
Jun 1, 2016·Frontiers in Plant Science·Md Amir HossainJan Zouhar
Jun 19, 2016·Trends in Plant Science·Kin Pan ChungLiwen Jiang
Apr 18, 2013·International Journal of Molecular Sciences·Yan DengStephen H Howell
Jun 28, 2013·International Journal of Molecular Sciences·Goretti Virgili-LópezAlessandro Vitale
Feb 8, 2018·Journal of Experimental Botany·Rengin OzgurIsmail Turkan
Mar 9, 2018·Frontiers in Plant Science·Ganesh M NawkarSang Yeol Lee
Apr 14, 2017·The Plant Journal : for Cell and Molecular Biology·Yuji IwataNozomu Koizumi
Nov 27, 2019·International Journal of Molecular Sciences·Péter PoórAttila Ördög
Mar 19, 2016·The New Phytologist·Jian-Xiang Liu, Stephen H Howell
Nov 3, 2020·Molecular Plant·Jing-Liang SunJian-Xiang Liu
Feb 19, 2020·Molecular Plant·Yanglin DingShuhua Yang
Nov 23, 2019·Trends in Biochemical Sciences·Noelia Pastor-CantizanoFederica Brandizzi
Jul 10, 2017·Seminars in Cell & Developmental Biology·Federica Brandizzi

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