Elevated activity of transcription factor nuclear factor of activated T-cells 5 (NFAT5) and diabetic nephropathy

Diabetes
Bingmei YangAndrew G Demaine

Abstract

The expression of aldose reductase is tightly regulated by the transcription factor tonicity response element binding protein (TonEBP/NFAT5) binding to three osmotic response elements (OREs; OREA, OREB, and OREC) in the gene. The aim was to investigate the contribution of NFAT5 to the pathogenesis of diabetic nephropathy. Peripheral blood mononuclear cells (PBMCs) were isolated from the following subjects: 44 Caucasoid patients with type 1 diabetes, of whom 26 had nephropathy and 18 had no nephropathy after a diabetes duration of 20 years, and 13 normal healthy control subjects. In addition, human mesangial cells (HMCs) were isolated from the normal lobe of 10 kidneys following radical nephrectomy for renal cell carcinoma. Nuclear and cytoplasmic proteins were extracted from PBMCs and HMCs and cultured in either normal or high-glucose (31 mmol/l D-glucose) conditions for 5 days. NFAT5 binding activity was quantitated using electrophoretic mobility shift assays for each of the OREs. Western blotting was used to measure aldose reductase and sorbitol dehydrogenase protein levels. There were significant fold increases in DNA binding activities of NFAT5 to OREB (2.06 +/- 0.03 vs. 1.33 +/- 0.18, P = 0.033) and OREC (1.94 +/- 0.21 vs....Continue Reading

References

Mar 11, 1992·The American Journal of Physiology·F L SmardoA Garcia-Perez
Aug 2, 1996·The Journal of Biological Chemistry·J D FerrarisA García-Pérez
Mar 1, 1997·Journal of the American Society of Nephrology : JASN·H N Ibrahim, T H Hostetter
May 12, 1998·The American Journal of Physiology·H MiyakawaH M Kwon
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MiyakawaH M Kwon
Mar 10, 1999·The American Journal of Physiology·J D FerrarisA García-Pérez
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Lopez-RodríguezA Rao
Jun 3, 2000·American Journal of Physiology. Renal Physiology·S K WooH M Kwon
Sep 21, 2000·Kidney International. Supplement·M Dunlop
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J TramaS N Ho
Jun 26, 2001·Kidney International·A D HodgkinsonA G Demaine
Aug 14, 2001·Virchows Archiv : an International Journal of Pathology·H KasajimaS Yagihashi
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Ohnn NahmH Moo Kwon
Dec 18, 2001·Diabetes/metabolism Research and Reviews·S H Hansen
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Djikolngar MaouyoHyug M Kwon
Dec 28, 2002·Diabetes Care·UNKNOWN Expert Committee on the Diagnosis and Classification of Diabetes Mellitus
Jan 23, 2003·Journal of the American Society of Nephrology : JASN·Ki Young NaH Moo Kwon

❮ Previous
Next ❯

Citations

Apr 29, 2009·Expert Reviews in Molecular Medicine·Amber Paratore Sanchez, Kumar Sharma
Mar 4, 2014·PloS One·Hongtai ZhangGuangming Zhou
May 24, 2012·Mediators of Inflammation·Christoph KüperWolfgang Neuhofer
Oct 27, 2015·BioMed Research International·Harald SeegerStephan Segerer
May 11, 2016·Scientific Reports·Soo Youn ChoiHyug Moo Kwon
Jul 16, 2013·Acta Diabetologica·Eliana D'AraioAndrea Hodgkinson
Oct 6, 2020·Journal of Diabetes Research·Lusha CenYi Cao
Mar 16, 2019·Frontiers in Immunology·Naeun LeeWan-Uk Kim
Jun 11, 2009·Physiology·Min Seong KwonH Moo Kwon
May 7, 2019·Frontiers in Immunology·Eun Jin YooSoo Youn Choi

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.