PMID: 9437407Jan 23, 1998Paper

Elevated apoptotic cell population in patients with chronic fatigue syndrome: the pivotal role of protein kinase RNA

Journal of Internal Medicine
A VojdaniC W Lapp

Abstract

A prominent feature of chronic fatigue syndrome (CFS) is a disordered immune system. Recent evidence indicates that induction of apoptosis might be mediated in a dysregulated immune system by the upregulation of growth inhibitory cytokines. Therefore, the purpose of this study was to evaluate the apoptotic cell population, interferon-alpha (IFN-alpha) and the IFN-induced protein kinase RNA (PKR) gene transcripts in peripheral blood lymphocytes (PBL) of CFS individuals, as compared to healthy controls. PBL were isolated from CFS (n = 29) and healthy control individuals (n = 15) and subjected to quantitative analysis of apoptotic cell population and cell cycle progression by flow cytometry. Quantitative competitive polymerase chain reaction (Q/C PCR) and Western blot analysis were used to assess the levels of PKR mRNA and protein in control and CFS individuals. In addition, circulating IFN-alpha was measured by ELISA assay. Increased apoptotic cell population was observed in CFS individuals, as compared to healthy controls (26.6 +/- 12.9% and 9.9 +/- 4.2%, respectively). The increased apoptotic subpopulation in CFS individuals was accompanied by an abnormal cell arrest in the S phase and the G2/M boundary of the cell cycle as com...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·R SchreckP A Baeuerle
Aug 15, 1992·Annals of Internal Medicine·A SchluederbergD Buchwald
Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Apr 1, 1992·Journal of the Royal Society of Medicine·S Wessely
Dec 1, 1992·Journal of Cellular Physiology·Q SongM F Lavin
Apr 3, 1992·Cell·G I EvanD C Hancock
Apr 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L E Gerschenson, R J Rotello
Jun 1, 1992·Trends in Pharmacological Sciences·W BurschR Schulte-Hermann
Mar 1, 1991·Clinical and Experimental Immunology·L J MorrisonP O Behan
Jan 1, 1991·Reviews of Infectious Diseases·A L Komaroff, D Buchwald
Jun 1, 1990·Journal of Clinical Microbiology·N G KlimasM A Fletcher
Oct 1, 1988·Molecular and Cellular Biology·R K TiwariG C Sen
Dec 1, 1989·Journal of Interferon Research·A G Hovanessian
Aug 7, 1989·The Medical Journal of Australia·W GinJ B Peter
Aug 7, 1989·The Medical Journal of Australia·A R LloydJ M Dwyer
May 29, 1981·Biochemical and Biophysical Research Communications·D FerbusM N Thang
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E F MeursA G Hovanessian
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·A KumarB R Williams
Jan 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R J SuhadolnikW A Carter
Jan 17, 1993·Biochimica Et Biophysica Acta·Y OnishiH Kizaki
Feb 1, 1996·The Journal of Infectious Diseases·C M SwaninkJ W van der Meer
Oct 1, 1995·Current Opinion in Immunology·D R Green, S J Martin
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·D L Vaux, A Strasser
Jul 1, 1996·The Journal of Cell Biology·S GrimmK Schulze-Osthoff
Feb 1, 1996·Current Opinion in Neurobiology·A FraserG I Evan

❮ Previous
Next ❯

Citations

Nov 26, 1998·Integrative Physiological and Behavioral Science : the Official Journal of the Pavlovian Society·D M SeeJ Tilles
Jul 17, 2012·Cell Stress & Chaperones·Philip L HooperPaul L Hooper
Aug 23, 2003·Journal of Psychosomatic Research·Marc LyallSimon Wessely
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·O SavinovaR Jagus
Jun 5, 1999·Journal of Internal Medicine·A Vojdani
Jan 1, 2003·Archives of Environmental Health·Jack D ThrasherAlan Broughton
Aug 12, 2014·PloS One·Wilfred C de VegaPatrick O McGowan
Jan 8, 2000·Annals of Behavioral Medicine : a Publication of the Society of Behavioral Medicine·S K JohnsonB H Natelson
Jul 25, 2000·Redox Report : Communications in Free Radical Research·R S RichardsH L Butt
Oct 2, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R PowellJ Almond
Jul 16, 2002·The Journal of Biological Chemistry·Edith DemettreBernard Lebleu
Aug 5, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S L Tan, M G Katze
Apr 5, 2001·Sports Medicine·R J Shephard
Sep 1, 1997·Toxicology and Industrial Health·A VojdaniP Choppa
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·Benjamin H NatelsonNicholas M Ponzio
Aug 28, 2007·Experimental Biology and Medicine·Suzanne H NiblettT B Rothkirch
Jun 30, 2019·Journal of Translational Medicine·Tiansong YangTetsuya Asakawa
May 10, 2002·Annals of the New York Academy of Sciences·R Patarca
May 13, 1999·Current Opinion in Rheumatology·D Buskila

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

The New England Journal of Medicine
C Calabrese, M Frederick
Epigenetics : Official Journal of the DNA Methylation Society
Beatriz Suárez-ÁlvarezCarlos López-Larrea
Epigenetics : Official Journal of the DNA Methylation Society
Degui ZhiDonna K Arnett
© 2021 Meta ULC. All rights reserved