Elevated cell invasion in a tumor sphere culture of RSV-M mouse glioma cells

Neurologia Medico-chirurgica
Motonobu NonakaTetsuya Ueba

Abstract

Cancer stem cells (CSCs) are the sole population possessing high self-renewal activity in tumors, with their existence affecting tumor recurrence. However, the invasive activity of CSCs has yet to be fully understood. In this article, we established a tumor sphere culture of RSV-M mouse glioma cells (RSV-M-TS) and evaluated their migration and invasion activities. Histological analysis of a tumor formed by cranial injection of the RSV-M-TS cells showed highly invasive properties and similarities with human malignant glioma tissues. When the migration activity of both RSV-M and RSV-M-TS cells were compared by intracranial injection, rapid migration of RSV-M-TS cells was observed. To confirm the invasive capabilities of RSV-M-TS cells, a three-dimensional collagen invasion assay was performed in vitro using RSV-M, RSV-M-TS, and RSV-M-TS cells cultured with medium containing serum. RSV-M and RSV-M-TS cultured with medium containing serum for 8 days indicated low migration activity, while moderate invasion activity was observed in RSV-M-TS cells. This activity was further enhanced by incubation with medium containing serum overnight. To identify the genes involved in this invasion activity, we performed quantitative polymerase chai...Continue Reading

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Citations

Mar 17, 2016·Human Pathology·Chengjin ZhaoJianbing Ren
May 31, 2019·Journal of Neuro-oncology·Toshio YawataTetsuya Ueba

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Methods Mentioned

BETA
surgical resection
electrophoresis
profiler
PCR

Software Mentioned

ABI Prism 7900 SDS

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