Elevated connexin-43 expression in term human myometrium correlates with elevated c-Jun expression and is independent of myometrial estrogen receptors

The Journal of Clinical Endocrinology and Metabolism
E GeimonenJ Andersen

Abstract

Just previous to the onset of parturition, a number of genes such as the one that codes for connexin-43 (Cx43) gap junction protein are induced in the myometrium. We have shown previously that activation of protein kinase C in human myometrial cultured cells leads to an up-regulation of cx43 transcription through an activating protein-1 element in the 5'-flanking promoter. Analyses were now performed on extracts of term myometrial tissue to test for an association between the up-regulation of cx43 expression and the expression of transcription factors and steroid hormone receptors that might regulate cx43 expression at term. Immunoblot analyses were performed on extracts of term myometrial tissue from women receiving elective or indicated cesarean sections to test for an association between the up-regulation of cx43 expression and the up-regulation of expression of the transcription factors c-Jun, c-Fos, and Sp1, which have cognate binding elements in the cx43 5'-flanking promoter. Immunoblot analysis, immunohistochemistry, and receptor binding assays were also performed to analyze the levels of progesterone receptors (PR) and estrogen receptors (ER) in the same term myometrial tissue, and these were compared to the levels in n...Continue Reading

References

Nov 1, 1979·Biology of Reproduction·R E GarfieldE E Daniel
Jul 1, 1979·The Journal of Clinical Endocrinology and Metabolism·G Giannopoulos, D Tulchinsky
Sep 1, 1992·Molecular Reproduction and Development·E M HendrixW J Larsen
Feb 1, 1990·The Journal of Cell Biology·B RisekN B Gilula
Nov 1, 1991·Molecular Endocrinology·Y Wang, R J Miksicek
Jul 1, 1990·Molecular Endocrinology·A WeiszF Bresciani
Aug 17, 1985·Lancet·R Garfield
Nov 4, 1988·Cell·T Curran, B R Franza
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y BerthoisB S Katzenellenbogen
Apr 1, 1986·Canadian Journal of Physiology and Pharmacology·L W Mackenzie, R E Garfield
Jan 1, 1982·American Journal of Obstetrics and Gynecology·R E GarfieldA I Csapo
Jul 1, 1982·Journal of Reproduction and Fertility·D C Wathes, D G Porter
Mar 1, 1980·American Journal of Obstetrics and Gynecology·C LevyE E Baulieu
Mar 1, 1980·The American Journal of Physiology·R E GarfieldE E Daniel
Jul 14, 1995·The Journal of Biological Chemistry·M Karin
Mar 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·M Pulkkinen, M M Hämäläinen
Apr 14, 1995·The Journal of Biological Chemistry·S M HyderG M Stancel
Jan 1, 1994·Critical Reviews in Eukaryotic Gene Expression·S M HyderD S Loose-Mitchell
Jun 1, 1994·Journal of Cellular Biochemistry·J P LandersT C Spelsberg
Nov 1, 1993·American Journal of Obstetrics and Gynecology·J AndersenP R Brink
May 1, 1993·American Journal of Obstetrics and Gynecology·J BalducciA M Vintzileos
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Mar 25, 1996·Molecular and Cellular Endocrinology·A Salmi, F M Rutanen
Jan 1, 1996·Annual Review of Biochemistry·D A GoodenoughD L Paul

❮ Previous
Next ❯

Citations

Nov 13, 2008·Physiological Genomics·Gustavo HelgueraEnrico Stefani
Sep 1, 2012·Molecular Endocrinology·Gary L Firestone, Bhumika J Kapadia
May 3, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Ratana Lim, Martha Lappas
Nov 13, 2008·The Journal of Clinical Endocrinology and Metabolism·James T SharkeyJames Olcese
Sep 24, 2015·PloS One·Alban LongchampJacques-Antoine Haefliger
Jul 24, 2002·Biology of Reproduction·Jennifer CondonWilliam E Rainey
Feb 6, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David A MacIntyrePhillip R Bennett
Jun 7, 2017·Biomedical Reports·Xiao-Fei Zhang, Xiaofeng Cui
May 14, 2019·Journal of Pregnancy·Othman A Al-ShboulMohammad Jaafar
Dec 30, 2021·Physiology·Elizabeth A Stewart, Romana A Nowak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.