Elevated cutaneous leukocyte concentration in a rodent model of acute venous hypertension

The Journal of Surgical Research
S G LalkaJ C Nixon

Abstract

The pathophysiologic mechanism of the skin pathology in chronic venous insufficiency is venous hypertension (VHTN). Microvascular dysfunction involving leukocytes has recently been proposed as the primary mediator of tissue damage from VHTN. We developed a rodent model allowing the investigation of the effects of acute VHTN on tissue leukocyte concentration. Under general anesthesia, adult male rats underwent transperitoneal isolation of the inferior vena cava and the common iliac veins and arteries. Bilateral thigh incisions allowed isolation of the common femoral veins and superficial epigastric veins (SEV: distal branch of the femoral vein in the thigh). Pressure in the SEV and flow in the iliac artery were measured before (T-Pre), immediately after (T-0), and for 135 min (T-1) after ligation of the cava, iliac, and femoral veins. Sham rats were identical except no venous ligation was performed. After the T-1 pressures were obtained, the distal hindlimb and forelimb skin was harvested and processed to measure myeloperoxidase (MPO) activity, an index of the number of tissue leukocytes. To evaluate the effect of arterial flow reduction known to occur with acute venous ligation, the above measurements were made in an Aortic gro...Continue Reading

References

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Citations

Aug 4, 2006·The New England Journal of Medicine·John J BerganBo Eklof
May 9, 2007·Annals of Vascular Surgery·John Bergan
Oct 12, 2012·Annals of the New York Academy of Sciences·Paritosh SumanMark A Hardy
Jan 16, 1999·The Journal of Surgical Research·T L HahnS G Lalka
Jan 8, 2008·Journal of Vascular Surgery·John J BerganGeert W Schmid-Schönbein
Sep 14, 2016·The Journal of Surgical Research·Paolo ZamboniPaola Secchiero
Jun 21, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anja FeldnerThomas Korff
Sep 29, 2019·Free Radical Biology & Medicine·Oliver Ta LyonsAlberto Smith
Jul 21, 2021·International Angiology : a Journal of the International Union of Angiology·Fatima Z CyrinoEliete Bouskela

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