Elevated Expression of AXL May Contribute to the Epithelial-to-Mesenchymal Transition in Inflammatory Bowel Disease Patients

Mediators of Inflammation
Éva BorosIstván Nagy

Abstract

Understanding the molecular mechanisms inducing and regulating epithelial-to-mesenchymal transition (EMT) upon chronic intestinal inflammation is critical for understanding the exact pathomechanism of inflammatory bowel disease (IBD). The aim of this study was to determine the expression profile of TAM family receptors in an inflamed colon. For this, we used a rat model of experimental colitis and also collected samples from colons of IBD patients. Samples were taken from both inflamed and uninflamed regions of the same colon; the total RNA was isolated, and the mRNA and microRNA expressions were monitored. We have determined that AXL is highly induced in active-inflamed colon, which is accompanied with reduced expression of AXL-regulating microRNAs. In addition, the expression of genes responsible for inducing or maintaining mesenchymal phenotype, such as SNAI1, ZEB2, VIM, MMP9, and HIF1α, were all significantly induced in the active-inflamed colon of IBD patients while the epithelial marker E-cadherin (CDH1) was downregulated. We also show that, in vitro, monocytic and colonic epithelial cells increase the expression of AXL in response to LPS or TNFα stimuli, respectively. In summary, we identified several interacting genes a...Continue Reading

References

Sep 26, 1991·The New England Journal of Medicine·D K Podolsky
Jun 2, 2006·Cytokine & Growth Factor Reviews·Sassan Hafizi, Björn Dahlbäck
Apr 19, 2008·Nature Reviews. Immunology·Greg Lemke, Carla V Rothlin
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christine GjerdrumJames B Lorens
Mar 23, 2010·Cell·Sergei I GrivennikovMichael Karin
Dec 15, 2010·Nature Reviews. Immunology·Maya Saleh, Giorgio Trinchieri
Jun 17, 2011·Nature·Bernard KhorRamnik J Xavier
Sep 22, 2011·Molecular Cancer Therapeutics·Anupam VermaSunil Sharma
Oct 24, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lauren Averett ByersJohn V Heymach
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lidia BosurgiCarla V Rothlin
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Mar 7, 2014·Blood·Jonathan H M van der MeerCornelis van 't Veer
Aug 16, 2014·Cancer Research·Catherine WilsonJeff Settleman
Sep 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Erinn B RankinAmato J Giaccia
Sep 10, 2014·Nature Immunology·Anna ZagórskaGreg Lemke
Sep 10, 2014·BioMed Research International·Thian-Sze WongJimmy Yu-Wai Chan
Oct 22, 2014·Molecular and Cellular Biology·Afnan Abu-ThuraiaJean-François Côté
Jan 9, 2015·Nature Reviews. Cancer·Douglas K GrahamH Shelton Earp
Feb 18, 2015·Scandinavian Journal of Gastroenterology·Johan Burisch, Pia Munkholm
Apr 11, 2015·Trends in Pharmacological Sciences·Gong Peng, Yang Liu
Sep 7, 2016·Cell Communication and Signaling : CCS·Stanley G KimaniRaymond B Birge
Jan 11, 2017·British Journal of Cancer·Carl M GayLauren Averett Byers
Dec 17, 2017·Molecular Therapy. Nucleic Acids·Pinar KanlikilicerGabriel Lopez-Berestein
Jan 28, 2016·Signal Transduction and Targeted Therapy·Yong Peng, Carlo M Croce
Jan 31, 2018·Nature Reviews. Immunology·Koji Taniguchi, Michael Karin

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Methods Mentioned

BETA
biopsies
PCR

Software Mentioned

Axiovision
SPSS

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