Elevated expression of proteoglycans in proliferating vascular smooth muscle cells

Atherosclerosis
Z TaoP Vijayagopal

Abstract

Smooth muscle cell (SMC) proliferation and increased production of arterial wall proteoglycans (PG) are implicated in atherogenesis. We investigated the effect of SMC proliferation on the biosynthesis of PG and the ability of the newly synthesized PG to bind low density lipoprotein (LDL). Proliferating and quiescent human aortic SMC were pulsed with [35S]sulfate for 24 h. Secreted and cell-associated PG were then analyzed. When SMC plated at a low density were induced to proliferate, PG synthesis increased significantly in comparison with quiescent cells. This was the net result of a 2.7-fold increase in secreted PG and a 1.3-fold increase in cell-associated PG. The increased PG synthesis in proliferating SMC correlated with a significant increase in the steady-state level of mRNA for perlecan and biglycan, and a modest increase in the versican-specific mRNA. The mRNA for decorin showed a 40% decrease. The increased PG secretion in proliferating cultures was due to increases in heparan sulfate PG, dermatan sulfate PG, and chondroitin sulfate PG secretion. Quiescent SMC at confluency produced 50% less PG than the corresponding SMC plated at a low density. Although confluent SMC stimulated to proliferate also had increased PG syn...Continue Reading

References

Apr 18, 1975·Biochimica Et Biophysica Acta·S R SrinivasanP Dolan
Aug 1, 1977·Experimental and Molecular Pathology·K D Curwen, S C Smith
Aug 1, 1992·Matrix : Collagen and Related Research·H M Walker-CaprioglioL J McGuffee
Jan 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J K ChenW L McKeehan
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T N Wight
Mar 1, 1987·Biological Chemistry Hoppe-Seyler·A SchmidtE Buddecke
Nov 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M W Lark, T N Wight
Mar 29, 1985·Biochimica Et Biophysica Acta·P VijayagopalG S Berenson
Apr 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A SchmidtE Buddecke
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·W D Wagner, S R Nohlgren
May 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·K J Williams, I Tabas
Jan 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·L E Cardoso, P A Mourão

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Citations

Aug 29, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Anastasia Z KaleaDorothy J Klimis-Zacas
Sep 16, 2000·European Journal of Pharmacology·E M DenholmP J Silver
Nov 10, 2009·Progress in Lipid Research·R TakechiJ C L Mamo
Nov 7, 2003·Journal of Cellular Physiology·Julio E FigueroaParakat Vijayagopal
Oct 22, 2004·Molecular & Cellular Proteomics : MCP·Estelle M FachGregory J Opiteck
Apr 2, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Amandine JaulmesMichel Raymondjean

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