Abstract
The cyclin-dependent protein kinase Pho85 is a known negative regulatory factor for two stress response genes, PHO5 and GSY2, which encode the inducible form of acid phosphatase and glycogen synthase, respectively, in the yeast Saccharomyces cerevisiae. Cells carrying a disruption of the PHO85 gene inappropriately express both PHO5 and GSY2, resulting in the increase in phosphate scavenging and hyperaccumulation of glycogen in nutrient-rich conditions. Constitutive activation of PKA in a pho85 mutant suppresses the hyperaccumulation of glycogen. This work presents data to show that, at least in part, the suppression of glycogen biosynthesis upon activation of PKA in a pho85 mutant results from the suppression of GSY2 expression. In addition to GSY2, disruption of the PHO85 gene inappropriately triggers the derepression of two other stress response genes, HSP12 and UBI4. At least in the case of GSY2, regulation of transcription by Pho85 is not through the stress-responsive cis-promoter elements (STRE). Furthermore, Pho85 may associate with the known cyclin Pho80 in the transcriptional regulation of these genes.
Citations
Jan 12, 2001·FEMS Microbiology Reviews·J François, J L Parrou
Feb 20, 2002·Trends in Biochemical Sciences·Adam S Carroll, Erin K O'Shea
Nov 13, 2001·Molecular Microbiology·H J McBrideD J Stillman
Jul 3, 1998·The EMBO Journal·A SmithS Garrett
Jul 22, 1998·The EMBO Journal·H J Bussink, S A Osmani
May 22, 2001·Molecular and Cellular Biology·N SchierR Fischer
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·A S CarrollE K O'Shea
Jul 21, 2015·FEBS Letters·Dae-Gwan Yi, Won-Ki Huh
Jun 10, 2008·Fungal Genetics and Biology : FG & B·Paula Fagundes de GouvêaGustavo Henrique Goldman
Nov 26, 2005·The EMBO Journal·Valeria WankeClaudio De Virgilio
Sep 14, 2007·Molecular Microbiology·Dongqing HuangBrenda Andrews
Jun 11, 2011·FEMS Microbiology Reviews·Claudio De Virgilio
Apr 24, 2010·FEMS Microbiology Reviews·Wayne A WilsonJavier Pozueta-Romero
Sep 16, 2008·PloS One·Nataly KacherovskyElton T Young
Jul 26, 2011·Enzyme Research·Elena V SambukMarina V Padkina
Mar 17, 2017·Microbiology and Molecular Biology Reviews : MMBR·Arsa ThammahongRobert A Cramer
Oct 9, 1999·Yeast·J L ParrouJ François
Apr 12, 2019·PloS One·Pham Thi Kim LienKenji Irie
Jun 29, 2004·Molecular Genetics and Genomics : MGG·B EnjalbertJ François
Jan 5, 2002·Canadian Journal of Microbiology·A E McDonaldW C Plaxton
Oct 6, 2000·Microbiology·B EnjalbertJ François
Jul 24, 1998·Science·N S GrayP G Schultz